Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Major histocompatibility complex class II- fetal skin dendritic cells are potent accessory cells of polyclonal T-cell responses
Autore:
Elbe-Burger, A; Mommaas, AM; Prieschl, EE; Fiebiger, E; Baumruker, T; Stingl, G;
Indirizzi:
Univ Vienna, Sch Med, VIRCC, Dept Dermatol,Div Immunol Allergy & Infect Dis, Vienna, Austria Univ Vienna Vienna Austria mmunol Allergy & Infect Dis, Vienna, Austria Leiden Univ, Med Ctr, Electron Microscopy Lab, Leiden, Netherlands Leiden Univ Leiden Netherlands tron Microscopy Lab, Leiden, Netherlands Novartis Res Inst, Dept Allerg Dis, Vienna, Austria Novartis Res Inst Vienna Austria Inst, Dept Allerg Dis, Vienna, Austria
Titolo Testata:
IMMUNOLOGY
fascicolo: 2, volume: 101, anno: 2000,
pagine: 242 - 253
SICI:
0019-2805(200010)101:2<242:MHCCIF>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTI-CD3 MONOCLONAL-ANTIBODIES; RECEPTOR CD3 COMPLEX; HUMAN LYMPHOCYTES-T; MHC CLASS-I; CO-STIMULATION; COSTIMULATORY SIGNALS; LYMPHOKINE PRODUCTION; ACTIVATION PATHWAYS; LANGERHANS CELLS; OKT3 ANTIBODY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
64
Recensione:
Indirizzi per estratti:
Indirizzo: Elbe-Burger, A Univ Vienna, Sch Med, VIRCC, Dept Dermatol,Div Immunol Allergy & Infect Dis, Vienna, Austria Univ Vienna Vienna Austria & Infect Dis, Vienna, Austria
Citazione:
A. Elbe-Burger et al., "Major histocompatibility complex class II- fetal skin dendritic cells are potent accessory cells of polyclonal T-cell responses", IMMUNOLOGY, 101(2), 2000, pp. 242-253

Abstract

Whereas dendritic cells (DC) and Langerhans cells (LC) isolated from organs of adult individuals express surface major histocompatibility complex (MHC) class II antigens, DC lines generated from fetal murine skin, while capable of activating naive, allogeneic CD8(+) T cells in a MHC class I-restricted fashion, do not exhibit anti-MHC class II surface reactivity and fail to stimulate the proliferation of naive, allogeneic CD4(+) T cells. To test whether the CD45(+) MHC class I+ CD80(+) DC line 80/1 expresses incompetent, or fails to transcribe, MHC class II molecules, we performed biochemical and molecular studies using Western blot and polymerase chain reaction analysis. We found that 80/1 DC express MHC class II molecules neither at the protein nor at the transcriptional level. Ultrastructural examination of these cells revealed the presence of a LC-like morphology with indented nuclei, active cytoplasm, intermediate filaments and dendritic processes. In contrast to adult LC, no LC-specific cytoplasmic organelles (Birbeck granules) were present. Functionally, 80/1 DC in the presence, but not in the absence, of concanavalin A and anti-T-cell receptor monoclonal antibodies stimulated a vigorous proliferative response of naive CD4(+) and CD8(+) T cells. Furthermore, we found that the anti-CD3-induced stimulation of naive CD4(+) and CD8(+) T cells was critically dependent on the expression of Fc gamma R on 80/1 DC and that the requirement for co-stimulation depends on the intensity of T-cell receptor signalling.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 00:12:27