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Titolo:
Isolation of the dxr gene of Zymomonas mobilis and characterization of the1-deoxy-D-xylulose 5-phosphate reductoisomerase
Autore:
Grolle, S; Bringer-Meyer, S; Sahm, H;
Indirizzi:
KFA Julich GmbH, Forschungszentrum, Inst Biotechnol 1, D-52425 Julich, Germany KFA Julich GmbH Julich Germany D-52425 echnol 1, D-52425 Julich, Germany
Titolo Testata:
FEMS MICROBIOLOGY LETTERS
fascicolo: 1, volume: 191, anno: 2000,
pagine: 131 - 137
SICI:
0378-1097(20001001)191:1<131:IOTDGO>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
TERPENOID BIOSYNTHESIS; NONMEVALONATE PATHWAY; ISOPRENOID BIOSYNTHESIS; HETEROLOGOUS EXPRESSION; INHIBITOR; CLONING; PLANTS;
Keywords:
1-deoxy-D-xylulose 5-phosphate reductoisomerase; 2C-methyl-D-erythritol 4-phosphate pathway; isoprenoid biosynthesis; Zymomonas mobilis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Sahm, H KFA Julich GmbH, Forschungszentrum, Inst Biotechnol 1, Postfach 1913, D-52425 Julich, Germany KFA Julich GmbH Postfach 1913 Julich Germany D-52425 ich, Germany
Citazione:
S. Grolle et al., "Isolation of the dxr gene of Zymomonas mobilis and characterization of the1-deoxy-D-xylulose 5-phosphate reductoisomerase", FEMS MICROB, 191(1), 2000, pp. 131-137

Abstract

The gene encoding the second enzyme of the 2C-methyl-D-erythritol 4-phosphate (MEP) pathway for isopentenyl diphosphate biosynthesis, 1-deoxy-D-xylulose 5-phosphate (DXP) reductoisomerase, was cloned and sequenced from Zymomonas mobilis. The deduced amino acid sequence showed the highest identity (48.2%) to the DXP reductoisomerase of Escherichia coli. Biochemical characterization of the purified DXP reductoisomerase showed a strict dependence of the enzyme on NADPH and divalent cations (Mn2+, Co2+ or Mg2+). The enzymeis a dimer with a molecular mass of 39 kDa per subunit and has a specific activity of 19.5 U mg protein(-1). Catalysis of the intramolecular rearrangement and reduction of DXP to MEP is competitively inhibited by the antibiotic fosmidomycin with a K-i of 0.6 mu.M. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 00:17:07