Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Blockade of the NMDA receptor increases developmental apoptotic elimination of granule neurons and activates caspases in the rat cerebellum
Autore:
Monti, B; Contestabile, A;
Indirizzi:
Univ Bologna, Dept Biol, I-40126 Bologna, Italy Univ Bologna Bologna Italy I-40126 na, Dept Biol, I-40126 Bologna, Italy
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 9, volume: 12, anno: 2000,
pagine: 3117 - 3123
SICI:
0953-816X(200009)12:9<3117:BOTNRI>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHRONIC NEONATAL BLOCKADE; D-ASPARTATE RECEPTOR; PROGRAMMED CELL-DEATH; ORNITHINE DECARBOXYLASE; NERVOUS-SYSTEM; ADULT-RAT; ALPHA-DIFLUOROMETHYLORNITHINE; DNA FRAGMENTATION; CYSTEINE PROTEASE; SURVIVAL;
Keywords:
cerebellar development; death proteases; granule cells; NMDA receptor antagonist; programmed cell death;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Contestabile, A Univ Bologna, Dept Biol, Via Belmeloro 8, I-40126 Bologna,Italy Univ Bologna Via Belmeloro 8 Bologna Italy I-40126 Italy
Citazione:
B. Monti e A. Contestabile, "Blockade of the NMDA receptor increases developmental apoptotic elimination of granule neurons and activates caspases in the rat cerebellum", EUR J NEURO, 12(9), 2000, pp. 3117-3123

Abstract

Elimination of neurons produced in excess naturally occurs during brain development through programmed cell death. Among the many survival factors affecting this process, a role for neurotransmitters acting on specific receptors has been suggested. We have performed an in vivo pharmacological blockade of the N-methyl-D-aspartate (NMDA) subtype of glutamate receptors, using the competitive NMDA receptor antagonist CGP 39551 at developmental stages corresponding to those at which a survival dependence on the stimulation of this receptor has been demonstrated for cerebellar granule neurons explanted in culture (typically from postnatal day 7 to postnatal day 11 or 13). We were able to demonstrate an increased level of DNA fragmentation in thecerebellum of the treated rats. At the P11 stage, in particular, the fragmented DNA extracted from the cerebellum of CGP 39551-treated pups showed a clear laddering of nucleosomal fragments after agarose-gel electrophoresis. Accordingly, in situ TUNEL technique showed a remarkable increase of cellspositive for nucleosomal DNA fragmentation, particularly in the inner granular layer of the cerebellum of treated rats at P11 stage. Therefore, the natural rate of apoptotic elimination of cerebellar granule neurons is considerably enhanced under conditions of pharmacological blockade of the NMDA receptor, thus demonstrating, for the first time in vivo, a clear survival dependence of these neurons upon the stimulation of the NMDA receptor. Concomitantly with the increased rate of apoptotic elimination of granule neurons, the activity of two death proteases of the caspase family, in particularof caspase 3 and caspase 1 at a lower extent, was remarkably increased in the cerebellum of the treated rats. On the contrary, a marker related to the normal differentiation process of granule neurons, the enzyme ornithine decarboxylase, was strongly decreased in its activity in the cerebellum of treated rat pups.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 07:40:52