Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ELISA versus immunolocalization to determine the association of Erwinia tracheiphila in Acalymma vittatum (Coleoptera : Chrysomelidae)
Autore:
Garcia-Salazar, C; Gildow, FE; Fleischer, SJ; Cox-Foster, D; Lukezic, FL;
Indirizzi:
Michigan State Univ, Dept Entomol, E Lansing, MI 48824 USA Michigan State Univ E Lansing MI USA 48824 tomol, E Lansing, MI 48824 USA Penn State Univ, Dept Plant Pathol, University Pk, PA 16802 USA Penn StateUniv University Pk PA USA 16802 l, University Pk, PA 16802 USA Penn State Univ, Dept Entomol, University Pk, PA 16802 USA Penn State Univ University Pk PA USA 16802 l, University Pk, PA 16802 USA
Titolo Testata:
ENVIRONMENTAL ENTOMOLOGY
fascicolo: 3, volume: 29, anno: 2000,
pagine: 542 - 550
SICI:
0046-225X(200006)29:3<542:EVITDT>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNOPEROXIDASE TECHNIQUES; BACTERIAL WILT; PSYCHODIDAE; MUSKMELON; MEMBRANE; INSECTS; DIPTERA;
Keywords:
Coleoptera; Chrysomelidae; Acalymma vittatum; vector-pathogen association; Erwinia tracheiphila;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Garcia-Salazar, C Michigan State Univ, Dept Entomol, E Lansing, MI 48824 USA Michigan State Univ E Lansing MI USA 48824 MI 48824 USA
Citazione:
C. Garcia-Salazar et al., "ELISA versus immunolocalization to determine the association of Erwinia tracheiphila in Acalymma vittatum (Coleoptera : Chrysomelidae)", ENV ENTOMOL, 29(3), 2000, pp. 542-550

Abstract

DAS-ELISA, immunohistochemistry and electron microscopy were used to investigate the association of the causal agent of bacterial wilt, Erwinia tracheiphila (Smith), within the beetle Acalymma vittatum (F.). After a 24-h acquisition period, a high percentage of individuals tested positive for E. tracheiphila antigen using both immunohistochemistry (100%) and DAS-ELISA (70-60%). Both assays showed that the antigen remained in beetles long after the initial acquisition, with the percentage declining during incubation. Using ELISA, the percentage decreased to 4.7% within 3 d after acquistion, then increased to 10% within 10 d and remained at 10% for 30 d. Immunoperoxidase assays of paraffin embedded gut sections were more sensitive, and showed that 95% of the beetles harbored the pathogen after 10 d and 20% after 30d. E. tracheiphila antigen was present throughout the digestive tract soonafter acquisition, but only small clusters of E. tracheiphila were otserved along the alimentary canal 3 d after transfer onto clean plants. After 10and 30 d on clean plants, E. tracheiphila antigen reaction was stronger and clusters of bacteria were more numerous. primarily in the posterior midgut and anterior portion of the hindgut. Scanning electron microscopy and TEMphotomicrographs confirmed the presence of bacterial cells resembling E. tracheiphila associated with the intima of the hindgut 1 and 30 d after acquisition. This demonstrated the sensitivity of immunohistochemistry for detecting E. tracheiphila within its vector, and suggests a long-term extracellular endosymbiotic association of E. tracheiphila with the alimentary canalof A. vittatum.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 20:43:25