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Titolo:
Chemoprevention of prostate carcinogenesis by alpha-difluoromethylornithine in TRAMP mice
Autore:
Gupta, S; Ahmad, N; Marengo, SR; MacLennan, GT; Greenberg, NM; Mukhtar, H;
Indirizzi:
Case Western Reserve Univ, Dept Dermatol, Cleveland, OH 44106 USA Case Western Reserve Univ Cleveland OH USA 44106 Cleveland, OH 44106 USA Case Western Reserve Univ, Dept Urol, Cleveland, OH 44106 USA Case WesternReserve Univ Cleveland OH USA 44106 Cleveland, OH 44106 USA Case Western Reserve Univ, Dept Pathol, Cleveland, OH 44106 USA Case Western Reserve Univ Cleveland OH USA 44106 Cleveland, OH 44106 USA Baylor Coll Med, Dept Cell Biol, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 ept Cell Biol, Houston, TX 77030 USA
Titolo Testata:
CANCER RESEARCH
fascicolo: 18, volume: 60, anno: 2000,
pagine: 5125 - 5133
SICI:
0008-5472(20000915)60:18<5125:COPCBA>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOLECULE E-CADHERIN; ORNITHINE DECARBOXYLASE; TRANSGENIC MOUSE; ANIMAL-MODELS; BETA-CATENIN; POLYAMINE BIOSYNTHESIS; CLINICAL IMPLICATIONS; CANCER PREVENTION; BREAST-CANCER; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
61
Recensione:
Indirizzi per estratti:
Indirizzo: Mukhtar, H Case Western Reserve Univ, Dept Dermatol, 11100 Euclid Ave, Cleveland, OH 44106 USA Case Western Reserve Univ 11100 Euclid Ave Cleveland OH USA 44106
Citazione:
S. Gupta et al., "Chemoprevention of prostate carcinogenesis by alpha-difluoromethylornithine in TRAMP mice", CANCER RES, 60(18), 2000, pp. 5125-5133

Abstract

Development of effective chemopreventive agents for human consumption requires conclusive evidence of their efficacy in animal models that have relevance to human diseases. Transgenic adenocarcinoma mouse prostate (TRAMP) isan excellent model of prostate cancer that mimics progressive forms of human disease inasmuch as 100% of males develop histological PIN by 8-12 weeksof age that progress to adenocarcinoma with distant site metastases by 24-28 weeks of age. In these animals, ornithine decarboxylase (ODC) activity (>3-fold) as well as protein expression (>4-fold) was found to be markedly higher in the dorsolateral prostate as compared with the nontransgenic littermates, suggesting their suitability to determine the chemopreventive effect of alpha-difluoromethylornithine (DFMO), an enzyme-activated irreversibleinhibitor of ODC, against prostate cancer, Using male TRAMP mice, we studied the effect of oral consumption of DFMO on development of prostate carcinogenesis and surrogate end point biomarkers related to prostate cancer progression, In two independent experiments, each consisting of 8 animals on test, the cumulative incidence of prostatic cancer development at 28 weeks ofage in 16 untreated TRAMP mice was 100% (16 of 16), whereas 94% (15 of 16)and 69% (11 of 16) of the animals exhibited distant site metastases to lymph nodes and lungs, respectively. Oral consumption of 1% DFMO (w/v) in the drinking water to TRAMP mice from 8 to 28 weeks of age resulted in a significant decrease in (a) weight (59%) and volume (66%) of prostate, (b) genitourinary weight (63%), and (c) ODC enzyme activity (52%) in the dorsolateralprostate. Importantly, in none of the DFMO-fed TRAMP mice were any distantmetastases to lymph node and lungs observed. Furthermore, DFMO treatment resulted in the marked reduction in the protein expression of proliferation cell nuclear antigen, ODC, and probasin in the dorsolateral prostate. The protein expression of antimetastases markers, i.e,, E-cadherin and alpha- and beta-catenin, was found to be restored in DFMO-fed animals as compared with the non-DFMO-fed mice, These chemopreventive effects of DFMO were further confirmed by immunohistochemical analysis of the dorsolateral prostate. Histological analysis of the dorsolateral prostate of DFMO-fed animals displayed marginal epithelial stratification, a small number of cribriform structures, elongated hyperchromatic epithelial nuclei, and a significant increase in apoptotic index. Non-DFMO-fed animals, on the other hand, displayed extensive epithelial stratification with profound cribriform structures accompanied with marked thickening, remodeling, and hypercellularity of the fibromuscular stroma, In nontransgenic littermates fed with DFMO, no significant alterations in the above parameters were evident. These data demonstratethat ODC represents a promising and rational target for chemoprevention ofhuman prostate cancer and that TRAMP mice are excellent models for screening of novel drugs and chemopreventive regimens for potential human use.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 22:12:41