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Titolo:
Involvement of an extracellular protease in algicidal activity of the marine bacterium Pseudoalteromonas sp strain A28
Autore:
Lee, SO; Kato, J; Takiguchi, N; Kuroda, A; Ikeda, T; Mitsutani, A; Ohtake, H;
Indirizzi:
Hiroshima Univ, Dept Fermentat Technol, Hiroshima 7398527, Japan HiroshimaUniv Hiroshima Japan 7398527 Technol, Hiroshima 7398527, Japan Fukuyama Univ, Dept Marine BIotechnol, Fukuyama, Hiroshima 7290292, Japan Fukuyama Univ Fukuyama Hiroshima Japan 7290292 , Hiroshima 7290292, Japan
Titolo Testata:
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
fascicolo: 10, volume: 66, anno: 2000,
pagine: 4334 - 4339
SICI:
0099-2240(200010)66:10<4334:IOAEPI>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
GYMNODINIUM; CHATTONELLA; ALTEROMONAS; SYSTEM; GROWTH; JAPAN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Kato, J Hiroshima Univ, Dept Fermentat Technol, Hiroshima 7398527, Japan Hiroshima Univ Hiroshima Japan 7398527 Hiroshima 7398527, Japan
Citazione:
S.O. Lee et al., "Involvement of an extracellular protease in algicidal activity of the marine bacterium Pseudoalteromonas sp strain A28", APPL ENVIR, 66(10), 2000, pp. 4334-4339

Abstract

The marine bacterium Pseudoalteromonas sp, strain A28 was able to kill thediatom Skeletonema costatum strain NIES-324, The culture supernatant of strain A28 showed potent algicidal activity when it was applied to a paper disk placed on a lawn of S. costatum NIES324. The condensed supernatant, which was prepared by subjecting the A28 culture supernatant to ultrafiltrationwith a 10,000-M-w-cutoff membrane, showed algicidal activity, suggesting that strain A28 produced extracellular substances capable of killing S. costatum cells. The condensed supernatant was then found to have protease and DNase activities. Two Pseudoalteromonas mutants lacking algicidal activity, designated NH1 and NH2, were selected after N-methyl-N'-nitrosoguanidine mutagenesis, The culture supernatants of NH1 and NH2 showed less than 15% of the protease activity detected with the parental strain, A28, The protease was purified to homogeneity from A28 culture supernatants by using ion-exchange chromatography followed by preparative gel electrophoresis, Paper-diskassays revealed that the purified protease had potent algicidal activity. The purified protease had a molecular mass for 50 kDa, and the N-terminal amino acid sequence was determined to be Ala-Thr-Pro-Asn-Asp-Pro. The optimum pH and temperature of the protease were found to be 8.8 and 30 degrees C,respectively, by using succinyl-Ala-Ala-Pro-Phe-p-nitroanilide as a substrate. The protease activity was strongly inhibited by phenylmethylsulfonyl fluoride, diisopropyl fluorophosphate, antipain, chymostatin, and leupeptin,No significant inhibition was detected with EDTA, EGTA, phenanthroline or tetraethylenepentamine. These results suggest that Pseudoalteromonas sp, strain A28 produced an extracellular serine protease which was responsible for the algicidal activity of this marine bacterium.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 04:31:57