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Titolo:
Inhibition of ornithine decarboxylase by alpha-difluoromethylornithine induces apoptosis of HC11 mouse mammary epithelial cells
Autore:
Ploszaj, T; Motyl, T; Zimowska, W; Skierski, J; Zwierzchowski, L;
Indirizzi:
Agr Univ Warsaw, Dept Anim Physiol, Fac Vet Med, PL-02787 Warsaw, Poland Agr Univ Warsaw Warsaw Poland PL-02787 Vet Med, PL-02787 Warsaw, Poland Drug Inst, Flow Cytometry Lab, Warsaw, Poland Drug Inst Warsaw PolandDrug Inst, Flow Cytometry Lab, Warsaw, Poland Polish Acad Sci, Inst Genet & Anim Breeding, Jastrzebiec, Poland Polish Acad Sci Jastrzebiec Poland & Anim Breeding, Jastrzebiec, Poland
Titolo Testata:
AMINO ACIDS
fascicolo: 2, volume: 19, anno: 2000,
pagine: 483 - 496
SICI:
0939-4451(2000)19:2<483:IOODBA>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
L1210 LEUKEMIC-CELLS; POLYAMINE SYNTHESIS INHIBITOR; ANTIOXIDANT DEFENSE; OXIDATIVE STRESS; SPERMINE; BCL-2; INDUCTION; PREVENTS; GROWTH; DEATH;
Keywords:
amino acids; polyamines; ornithine decarboxylase; alpha-difluoromethylornithine; apoptosis; Bcl-2; mammary epithelial cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Ploszaj, T Agr Univ Warsaw, Dept Anim Physiol, Fac Vet Med, Nowoursynowska166, PL-02787 Warsaw, Poland Agr Univ Warsaw Nowoursynowska 166 Warsaw Poland PL-02787 land
Citazione:
T. Ploszaj et al., "Inhibition of ornithine decarboxylase by alpha-difluoromethylornithine induces apoptosis of HC11 mouse mammary epithelial cells", AMINO ACIDS, 19(2), 2000, pp. 483-496

Abstract

The effect of a-difluoromethylornithine (DFMO) on the apoptosis of HC11 mouse mammary epithelial cells was investigated. The involvement of reactive oxygen species (ROS) and Bcl-2 protein in the mechanism of apoptosis induced by ornithine decarboxylase (ODC) inhibition was also assessed. DFMO (0.1,1 and 5 mM) induced apoptosis of HC11 cells in dose- and time-dependent manner. Apoptosis manifests itself with morphological features like: cell shrinkage, condensation of chromatin, pyknosis and fragmentation of nucleus, followed by secondary necrosis (putrosis). The decrease in the nuclear DNA contents appearing as the hypodiploidal peak sub-G, in the DNA histogram wasnot dependent on the presence of prolactin (5 mu g/ml) in DFMO-treated cultures. Apoptosis induced by ODC inhibition was associated with a rapid increase in ROS concentration in HC11 cells observed within Ih after DFMO treatment. The down-regulation of Bcl-2 as a decrease in cell number expressing bcl-2 and a lowered Bcl-2 protein content in cells expressing this protooncogene was also noted. The administration of putrescine (50 mu M) lowered the number of early-apoptotic, late-apoptotic and necrotic cells. Moreover, it increased the number of cells expressing bcl-2. In conclusion, the disturbance of cellular polyamine homeostasis by inhibition of their synthesis enhances mammary epithelial cell susceptibility to apoptosis. It may occur inthe mammary gland at the end of lactation, when the depletion of circulating lactogenic hormones and activation of intra-mammary apoptogenic factors expression take place.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 12:05:41