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Titolo:
Follicular lymphoma can be distinguished from benign follicular hyperplasia by flow cytometry using simultaneous staining of cytoplasmic bcl-2 and cell surface CD20
Autore:
Cornfield, DB; Mitchell, DM; Almasri, NM; Anderson, JB; Ahrens, KP; Dooley, EO; Braylan, RC;
Indirizzi:
Univ Florida, Coll Med, Dept Pathol & Lab Med, Hematopathol Sect, Gainesville, FL 32610 USA Univ Florida Gainesville FL USA 32610 hol Sect, Gainesville, FL 32610 USA Univ Florida, Coll Med, Shands Hosp, Gainesville, FL 32610 USA Univ Florida Gainesville FL USA 32610 nds Hosp, Gainesville, FL 32610 USA
Titolo Testata:
AJCP. American journal of clinical pathology
fascicolo: 2, volume: 114, anno: 2000,
pagine: 258 - 263
Fonte:
ISI
Lingua:
ENG
Soggetto:
NON-HODGKINS-LYMPHOMA; PROGNOSTIC-SIGNIFICANCE; PROTEIN EXPRESSION; GENE; REARRANGEMENT; TRANSLOCATION; FOLLICLES; T(14-18); TISSUE;
Keywords:
flow cytometry; bcl-2; follicular lymphoma; follicular hyperplasia;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Braylan, RC Univ Florida, Coll Med, Dept Pathol & Lab Med, Hematopathol Sect, Box 100275, Gainesville, FL 32610 USA Univ Florida Box 100275 Gainesville FL USA 32610 FL 32610 USA
Citazione:
D.B. Cornfield et al., "Follicular lymphoma can be distinguished from benign follicular hyperplasia by flow cytometry using simultaneous staining of cytoplasmic bcl-2 and cell surface CD20", AM J CLIN P, 114(2), 2000, pp. 258-263

Abstract

The distinction between benign follicular hyperplasia (FH) and follicular lymphoma (FL) is sometimes problematic. We wanted to determine whether the expression of bcl-2 of FH was quantitatively different from that of FL, using surface CD20 expression as a discriminator of the various lymphoid compartments. Lymph node cell suspensions from 12 cases of FH and 17 cases of FLwere analyzed by flow cytometry using a combined surface CD20 and intracellular bcl-2 staining. CD20- T cells in FH demonstrated the same bcl-2 expression as the CD20+ mantle cells, but the bright CD20+ germinal center cellsshowed near absence of bcl-2 expression. In contrast, the neoplastic cellsof FL showed greater bcl-2 expression than the T cells of the same tumors and all cell populations of FH. This difference was particularly significant between the neoplastic B cells of FL and the germinal center cells of FH. The combined analysis of CD20 and bcl-2 should be useful for the differential diagnosis between FH and FL and particularly applicable to limited samples or when B-cell clonality is in question. Whether the quantitation of bcl-2 expression can be of further discriminatory value in malignant lymphomas remains to be determined.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 00:07:03