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Titolo:
Secretory transport of cadmium through intestinal brush border membrane via H+-antiport
Autore:
Endo, T; Kimura, O; Saitoh, H; Sakata, M;
Indirizzi:
Hlth Sci Univ Hokkaido, Fac Pharmaceut Sci, Tobetsu, Hokkaido 0610293, Japan Hlth Sci Univ Hokkaido Tobetsu Hokkaido Japan 0610293 aido 0610293, Japan
Titolo Testata:
TOXICOLOGY
fascicolo: 1-3, volume: 150, anno: 2000,
pagine: 129 - 136
SICI:
0300-483X(20000907)150:1-3<129:STOCTI>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
INORGANIC ANION-EXCHANGER; EPITHELIAL-CELL LINE; RAT SMALL-INTESTINE; LLC-PK1 CELLS; DEPENDENT TRANSPORT; APICAL MEMBRANE; ORGANIC CATION; BASOLATERAL MEMBRANES; ABSORPTION; VESICLES;
Keywords:
cadmium; secretion; pH; intestinal brush border membrane vesicles; Caco-2 cells; organic cation transporter;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Endo, T Hlth Sci Univ Hokkaido, Fac Pharmaceut Sci, 1757 Ishikari Tobetsu,Tobetsu, Hokkaido 0610293, Japan Hlth Sci Univ Hokkaido 1757 Ishikari Tobetsu Tobetsu Hokkaido Japan 0610293
Citazione:
T. Endo et al., "Secretory transport of cadmium through intestinal brush border membrane via H+-antiport", TOXICOLOGY, 150(1-3), 2000, pp. 129-136

Abstract

The effect of pH on the secretory transport of Cd through the intestinal brush border membrane was investigated using isolated rat intestinal brush border membrane vesicles (BBMV) and the Caco-2 intestinal epithelial cell line. BBMV equilibrated at pH 5.5 or 7.5 (pH(m)) were mixed with an experimental buffer at pH 5.5 or 7.5 (pH(out)) containing CdCl2. The initial accumulation of Cd in BBMV incubated for 1 or 3 min at pH(in) 5.5 and pH(out) 7.5 (outwardly directed H+-gradient) was significantly higher than that at pH(in) = pH(out) = 7.5, but the equilibrated Cd accumulation incubated for 30 min was marginally lower. Carbonylcyanide-p-trifluoromethoxyphenylhydrazone (FCCP), a protonophore, diminished the increasing effect of the H+-gradienton the initial Cd accumulation. Caco-2 cell monolayers cultured on permeable membranes were incubated with CdCl2 from the basolateral medium, and thetransport of Cd from the basolateral to apical medium and the accumulationof Cd in the monolayers were measured. Cd transport was increased by lowering the pH of the apical medium, and was accompanied by a decrease in the Cd accumulation. Coincubation with CdCl2 and tetraethylammonium, a typical, substrate for H+-antiport of the renal organic cation transporter, from thebasolateral medium slightly but significantly decreased the basolateral-to-apical transport of Cd, with a concomitant increase in the Cd accumulation. These findings suggest the secretory transport of Cd through the intestinal brush border membrane not only via passive diffusion but also via H+-antiport of the putative organic cation transporter. (C) 2000 Elsevier ScienceIreland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 12:37:37