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Titolo:
Hormonal regulation of sodium/sulfate co-transport in renal epithelial cells
Autore:
Lee, HJ; Sagawa, K; Shi, W; Murer, H; Morris, ME;
Indirizzi:
SUNY Buffalo, Sch Pharm, Dept Pharmaceut, Amherst, NY 14260 USA SUNY Buffalo Amherst NY USA 14260 Dept Pharmaceut, Amherst, NY 14260 USA Univ Zurich, Inst Physiol, CH-8057 Zurich, Switzerland Univ Zurich Zurich Switzerland CH-8057 siol, CH-8057 Zurich, Switzerland
Titolo Testata:
PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE
fascicolo: 1, volume: 225, anno: 2000,
pagine: 49 - 57
SICI:
0037-9727(200010)225:1<49:HROSCI>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
BORDER MEMBRANE-VESICLES; ALTERED DIETARY SULFATE; INORGANIC SULFATE; BASOLATERAL MEMBRANE; KIDNEY-CELLS; RAT; COTRANSPORT; QUANTITATION; ADAPTATION; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Morris, ME SUNY Buffalo, Sch Pharm, Dept Pharmaceut, 527 Hochstetter Hall,Amherst, NY 14260 USA SUNY Buffalo 527 Hochstetter Hall Amherst NY USA 14260 4260 USA
Citazione:
H.J. Lee et al., "Hormonal regulation of sodium/sulfate co-transport in renal epithelial cells", P SOC EXP M, 225(1), 2000, pp. 49-57

Abstract

Serum sulfate concentrations are elevated in infants, young children, and pregnant women due, at least in part, to increased renal sulfate reabsorption. Little is known about the effects of hormones, particularly those involved in growth, development, and pregnancy, on renal sulfate reabsorption. The objective of this investigation was to examine the effects of growth hormone (GH), insulin-like growth factor 1 (IGF-1), progesterone (PG), and 17 beta-estradiol (EST) on renal sodium/sulfate co-transport. S-35-sulfate uptake was determined in Madin-Darby canine kidney (MDCK)/NaSi-1 cells (MDCK cells that have been stably transfected with rat sodium/sulfate co-transporter (NaSi-1) cDNA) and in opossum kidney (OK) cells, NaSi-1 mRNA was determined by RT-PCR and protein levels by ELISA. GH (0.1 nM) significantly increased the sodium/sulfate co-transport In MDCK/NaSi-1 cells up to 35%. IGF-1 induced a concentration-related stimulation of the sodium/sulfate cc-transport with a maximal response observed at 1000 nM (59% increase). Sodium-dependent sulfate uptake was significantly increased when cells were preincubated with 10 nM PG, 10 nM EST, or 10 nM PG/10 nM EST up to 41%, 46%, or 39%, respectively, OK cells exhibited endogenous sodium-dependent sulfate transport; significantly increased sodium/sulfate co-transport was also observed in OK cells that were preincubated with GH, IGF-1, and PG/EST, although not with EST alone. The NaSi-1 mRNA and NaSi-1 protein levels were significantly increased in MDCK/NaSi-1 cells treated with 0.1 nM GH, 100 nM IGF-1, 10 nM PG, and/or 10 nM EST compared with control. These results suggest that the increased renal sulfate reabsorption that occurs in neonates, young and pregnant humans, and animals could be mediated by the increased steady-statelevels of NaSi-1 mRNA produced by the higher plasma concentrations of GH, IGF-1, or PG/EST.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 08:15:56