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Titolo:
Cytosolic heat-stress proteins Hsp17.7 class I and Hsp17.3 class II of tomato act as molecular chaperones in vivo
Autore:
Low, D; Brandle, K; Nover, L; Forreiter, C;
Indirizzi:
Univ Frankfurt, Biozentrum, D-60349 Frankfurt, Germany Univ Frankfurt Frankfurt Germany D-60349 rum, D-60349 Frankfurt, Germany Univ Frankfurt, Inst Zool, D-60054 Frankfurt, Germany Univ Frankfurt Frankfurt Germany D-60054 ool, D-60054 Frankfurt, Germany
Titolo Testata:
PLANTA
fascicolo: 4, volume: 211, anno: 2000,
pagine: 575 - 582
SICI:
0032-0935(200009)211:4<575:CHPHCI>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
SHOCK-PROTEIN; CDNA CLONE; DEPENDENT EXPRESSION; LOCALIZATION; GENES; PEA; CHLOROPLASTS; GERMINATION; SEQUENCE; GRANULES;
Keywords:
chaperone activity; heat stress; Lycopersicon; small stress proteins;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Forreiter, C Univ Frankfurt, Biozentrum, Marie Curie Str 9, D-60349 Frankfurt, Germany Univ Frankfurt Marie Curie Str 9 Frankfurt Germany D-60349 y
Citazione:
D. Low et al., "Cytosolic heat-stress proteins Hsp17.7 class I and Hsp17.3 class II of tomato act as molecular chaperones in vivo", PLANTA, 211(4), 2000, pp. 575-582

Abstract

Small heat-stress proteins (sHsps) are the most abundant stress-induced proteins with up to 20 different members in higher plants. In the cytoplasm, two different classes call be distinguished. Two cDNA clones from tomato Lycopersicon peruvianum (L. ) Mill., each coding for one of the cytoplasmic sHsp subfamilies, were analyzed with respect to their transcript and protein expression, genome organization and chaperone activity. Neither type was present under control conditions but both appeared upon heat stress and in mature fruits. Expression of the class II transcript was found to be induced at slightly lower temperatures than the class I transcript. Protein analysis using class-specific antibodies revealed an identical expression pattern of both corresponding proteins. Transient expression in an Arabodopsis thaliana (L. ) Heynh. cell culture showed that, despite the difference ill theiramino acid sequence, both classes are functionally active as chaperones invivo, as shown by their ability to prevent thermal inactivation of fireflyluciferase in a cellular environment.

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Documento generato il 22/10/20 alle ore 03:10:22