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Titolo:
A novel and direct assay for measuring enzymatic depolymerisation of beta-1,3-glucans
Autore:
Aktas, LY; Adlard, M; Trevan, M; Guven, A; Brownleader, MD;
Indirizzi:
Univ Westminster, Sch Biosci, London W1M 8JS, England Univ Westminster London England W1M 8JS Biosci, London W1M 8JS, England Ege Univ, Dept Biol, TR-35100 Izmir, Turkey Ege Univ Izmir Turkey TR-35100 e Univ, Dept Biol, TR-35100 Izmir, Turkey
Titolo Testata:
PHYTOCHEMICAL ANALYSIS
fascicolo: 5, volume: 11, anno: 2000,
pagine: 301 - 303
SICI:
0958-0344(200009/10)11:5<301:ANADAF>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
PURIFICATION;
Keywords:
beta-1,3-D-glucanase; laminarinase; beta-1,3-glucan; laminarin; polarimetry; Lycopersicon esculentum;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
8
Recensione:
Indirizzi per estratti:
Indirizzo: Brownleader, MD Univ Westminster, Sch Biosci, 115 New Cavendish St, LondonW1M 8JS, England Univ Westminster 115 New Cavendish St London England W1M8JS
Citazione:
L.Y. Aktas et al., "A novel and direct assay for measuring enzymatic depolymerisation of beta-1,3-glucans", PHYTOCH AN, 11(5), 2000, pp. 301-303

Abstract

Indirect colorimetric analyses of released reducing groups, as an indicator of induced beta-1,3-glucanase activities, tend to be the assay methods ofchoice for the characterization of plant endo- and exo-beta-1,3-glucanase,However, interfering low molecular weight reducing sugars found in unprocessed plant extracts often mask in vitro estimations of P-ld-glucanase activity. The enzyme-catalysed hydrolysis of an optically active beta-1,3-glucanpolymer was monitored polarimetrically by measuring changes in the rotation of plane-polarized light of the reaction assay medium. A direct, non-radiochemical assay that detects changes in the optical rotation of released (1->3) beta-D-oligoglucosides with low degrees of polymerisation (<<25) has been found to be highly reproducible, rapid (total analysis time 15 min), sensitive (detection limit 0.007 unit/mL glucanase), selective and non-destructive. This assay has been used to detect a salicylate-induced cell wall-bound tomato beta-1,3-glucanase, which is a component of a pathogenesis-related response in plants. Copyright (C) 2000 John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 11:16:13