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Titolo:
EVIDENCE FOR SURVIVAL AND METABOLIC-ACTIVITY OF ENCAPSULATED XENOGENEIC HEPATOCYTES TRANSPLANTED WITHOUT IMMUNOSUPPRESSION IN GUNN-RATS
Autore:
GOMEZ N; BALLADUR P; CALMUS Y; BAUDRIMONT M; HONIGER J; DELELO R; MYARA A; CREMA E; TRIVIN F; CAPEAU J; NORDLINGER B;
Indirizzi:
HOP ST ANTOINE,CTR CHIRURG DIGEST,ANAT PATHOL LAB,184 RUE FG ST ANTOINE F-75571 PARIS 12 FRANCE HOP ST ANTOINE,CTR CHIRURG DIGEST,ANAT PATHOL LAB F-75571 PARIS 12 FRANCE HOP ST ANTOINE,LAB RECH CHIRURG F-75571 PARIS 12 FRANCE HOP COCHIN,CLIN CHIRURG F-75674 PARIS FRANCE HOP ST JOSEPH,BIOCHIM LAB F-75014 PARIS FRANCE
Titolo Testata:
Transplantation
fascicolo: 12, volume: 63, anno: 1997,
pagine: 1718 - 1723
SICI:
0041-1337(1997)63:12<1718:EFSAMO>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
MICROCARRIER-ATTACHED HEPATOCYTES; HEPATOCELLULAR TRANSPLANTATION; LIVER-FUNCTION; CYCLOSPORINE; MICROENCAPSULATION; HYPERBILIRUBINEMIA; REPLACEMENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
N. Gomez et al., "EVIDENCE FOR SURVIVAL AND METABOLIC-ACTIVITY OF ENCAPSULATED XENOGENEIC HEPATOCYTES TRANSPLANTED WITHOUT IMMUNOSUPPRESSION IN GUNN-RATS", Transplantation, 63(12), 1997, pp. 1718-1723

Abstract

Background. Hepatocyte transplantation could be an alternative to whole organ transplantation to correct enzymatic disorders. To this end, it would be of major importance to use xenogeneic cells without immunosuppression. The aim of this study was to investigate the survival andmetabolic activity of encapsulated xenogeneic hepatocytes in the absence of immunosuppression. For this purpose, we used Gunn rats genetically incapable of bilirubin conjugation. Methods. Xenogeneic (from guinea pigs) and allogeneic (from Lewis rats) hepatocytes (2 x 10(7)) wereisolated, macroencapsulated in hydrogel hollow fibers made with an acrylonitrile-sodium methallyl-sulfonate copolymer, and transplanted into the peritoneum of Gunn rats without any immunosuppression. Plasma bilirubin levels were evaluated weekly, Bilirubin conjugates in bile andcell morphology were studied after 5 and 12 weeks, respectively. Results. In Gunn rats transplanted with xenogeneic hepatocytes, a significant decrease in the serum bilirubin level was observed between 3 and 9weeks after transplantation when compared with controls transplanted with empty hollow fibers: it fell to 62% of the initial level at weeks5-7 (P<0.01). A comparable result was observed in Gunn rats transplanted with encapsulated allogeneic cells, Bilirubin conjugates were observed in bile samples of rats transplanted with encapsulated hepatocytes, After explantation, hollow fibers appeared intact with minimal fibrosis. Cell viability and hepatocyte morphology were preserved. Conclusions. These results indicate that macroencapsulated xenogeneic hepatocytes can survive and remain functional for more than 2 months when transplanted in vivo in the absence of any immunosuppression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/21 alle ore 14:31:14