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Titolo:
Identification of an RNA element that confers post-transcriptional repression of connective tissue growth factor/hypertrophic chondrocyte specific 24(ctgf/hcs24) gene: Similarities to retroviral RNA-protein interactions
Autore:
Kubota, S; Kondo, S; Eguchi, T; Hattori, T; Nakanishi, T; Pomerantz, RJ; Takigawa, M;
Indirizzi:
Okayama Univ, Sch Dent, Dept Biochem & Mol Dent, Okayama 7008525, Japan Okayama Univ Okayama Japan 7008525 em & Mol Dent, Okayama 7008525, Japan Thomas Jefferson Univ, Jefferson Med Coll, Dept Med,Dorrance H Hamilton Labs, Ctr Human Virol,Div Infect Dis, Philadelphia, PA 19107 USA Thomas Jefferson Univ Philadelphia PA USA 19107 hiladelphia, PA 19107 USA
Titolo Testata:
ONCOGENE
fascicolo: 41, volume: 19, anno: 2000,
pagine: 4773 - 4786
SICI:
0950-9232(20000928)19:41<4773:IOARET>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANCHORAGE-INDEPENDENT GROWTH; VASCULAR ENDOTHELIAL-CELLS; ANGIOGENESIS IN-VIVO; CAP-BINDING-PROTEIN; MESSENGER-RNAS; FACTOR-BETA; NEGATIVE REGULATOR; NUCLEAR EXPORT; LINE HCS-2/8; REV PROTEIN;
Keywords:
CTGF; angiogenesis; chondrocyte; HIV-1 Rev; 3 '-UTR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Takigawa, M Okayama Univ, Sch Dent, Dept Biochem & Mol Dent, 2-5-1 ShikataCho, Okayama 7008525, Japan Okayama Univ 2-5-1 Shikata Cho Okayama Japan 7008525 5, Japan
Citazione:
S. Kubota et al., "Identification of an RNA element that confers post-transcriptional repression of connective tissue growth factor/hypertrophic chondrocyte specific 24(ctgf/hcs24) gene: Similarities to retroviral RNA-protein interactions", ONCOGENE, 19(41), 2000, pp. 4773-4786

Abstract

The repressive effect of the 3'-untranslated region (3'-UTR) in human connective tissue growth factor/hypertrophic chondrocyte specific 24 (ctgf/hcs24) mRNA on gene expression had been demonstrated in our previous study. Here, we identified a minimal RNA element in the 3'-UTR, which acts as a cis-acting element of structure-anchored repression (CAESAR). Deletion analyses of the 3'-UTR led us to minimize the element of 84 bases at the junction ofthe coding region and the 3'-UTR. The minimized RNA segment is predicted, and actually capable of forming a stable secondary structure in vitro. Mutational analyses disclosed a significant relationship between the predicted structure and repressive effect. The utility of CAESAR as a post-transcriptional regulatory element was represented by the fact that steady-state mRNAlevels were not affected by CAESAR linked in cis, while protein levels from such a chimeric gene were markedly reduced. Of note, the CAESAR sequence exerted no effect, when it was placed upstream of the promoter. Finally, RNA gel electromobility-shift analyses demonstrated a nuclear factor that interacts with the folded CAESAR. Taken together, it was uncovered that CAESARof ctgf is a novel post-transcriptional structured RNA regulatory element,probably acting through direct interactions with a nuclear factor as observed in retroviral RNA elements with certain proteins.

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Documento generato il 28/03/20 alle ore 23:14:11