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Titolo:
Directed axonal growth towards axolotl limb blastemas in vitro
Autore:
Tonge, DA; Leclere, PG;
Indirizzi:
Univ London Kings Coll, Ctr Res Neurosci, Neural Damage & Repair Grp, London SE1 1UL, England Univ London Kings Coll London England SE1 1UL p, London SE1 1UL, England
Titolo Testata:
NEUROSCIENCE
fascicolo: 1, volume: 100, anno: 2000,
pagine: 201 - 211
SICI:
0306-4522(2000)100:1<201:DAGTAL>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
SENSORY NEURONS; NEUROTROPHIC FACTOR; IN-VITRO; REGENERATING AXONS; DIFFUSIBLE FACTORS; PERIPHERAL-NERVES; AMPHIBIAN LIMB; SCIATIC-NERVE; EXPRESSION; RECEPTORS;
Keywords:
nerve growth factor; neurotrophin; fibroblast growth factor; trk receptors; axonal guidance; blastema;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Tonge, DA Univ London Kings Coll, Ctr Res Neurosci, Neural Damage & RepairGrp, GuysHosp Campus, London SE1 1UL, England Univ London Kings Coll Guys Hosp Campus London England SE1 1UL
Citazione:
D.A. Tonge e P.G. Leclere, "Directed axonal growth towards axolotl limb blastemas in vitro", NEUROSCIENC, 100(1), 2000, pp. 201-211

Abstract

Limb amputation in urodele amphibia is followed by formation of a blastema, which subsequently develops into a complete limb with normal pattern of innervation. In this study, we investigated the effects of axolotl limb blastemas on axonal growth in gels of collagen and extracellular matrix (matrigel). When peripheral nerves with attached dorsal root ganglia were culturedin collagen gels together with blastemas, axonal outgrowth was markedly increased compared with control preparations. Blastemas contain fibroblast growth factors, and may also contain neurotrophic factors such as nerve growth factor, brain-derived neurotrophic factor, neurotrophin 3, neurotrophin 4, glial cell line-derived neurotrophic factor and hepatocyte growth factor/scatter factor, since these factors are expressed in developing limbs in other vertebrates. In collagen gels the neurotrophins and glial cell line-derived neurotrophic factor stimulated axonal growth, but outgrowing axons were shorter than in co-cultures with blastemas. The tyrosine kinase inhibitorK252a blocked the stimulatory effects of the neurotrophins on axonal growth but had relatively little effect on axonal growth in co-cultures with blastemas. In experiments in which peripheral nerves, with attached dorsal root ganglia, were cultured in matrigel, axons grew towards blastemas over distances of about 1 mm. Directed axonal growth even occurred in these co-cultures after addition of high concentrations of all the above neurotrophic factors, suggesting that blastemas may release a different factor which stimulates axonal growth. The results indicate that during early stages of limb regeneration in amphibia, factor(s) are released which are capable of attracting the growth of peripheral nerves and may play an important role in the development of innervation of regenerated limbs. The identity of the factor(s) remains to be determined. (C) 2000 IBRO. Published by Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/06/20 alle ore 09:30:27