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Titolo:
Mmf1p, a novel yeast mitochondrial protein conserved throughout evolution and involved in maintenance of the mitochondrial genome
Autore:
Oxelmark, E; Marchini, A; Malanchi, I; Magherini, F; Jaquet, L; Hajibagheri, MAN; Blight, KJ; Jauniaux, JC; Tommasino, M;
Indirizzi:
Deutsch Krebsforschungszentrum, Abt F0200, D-69120 Heidelberg, Germany Deutsch Krebsforschungszentrum Heidelberg Germany D-69120 lberg, Germany Deutsch Krebsforschungszentrum, Abt F0100, INSERM, U375, D-69120 Heidelberg, Germany Deutsch Krebsforschungszentrum Heidelberg Germany D-69120 lberg, Germany Imperial Canc Res Fund, London WC2A 3PX, England Imperial Canc Res Fund London England WC2A 3PX London WC2A 3PX, England
Titolo Testata:
MOLECULAR AND CELLULAR BIOLOGY
fascicolo: 20, volume: 20, anno: 2000,
pagine: 7784 - 7797
SICI:
0270-7306(200010)20:20<7784:MANYMP>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
SACCHAROMYCES-CEREVISIAE; MOLECULAR CHAPERONES; HISTONE HM; DNA; PURIFICATION; MEMBRANE; HOMOLOG; NUCLEAR; GROWTH; BINDS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Tommasino, M Deutsch Krebsforschungszentrum, Abt F0200, Neuenheimer Feld 242, D-69120 Heidelberg, Germany Deutsch Krebsforschungszentrum Neuenheimer Feld 242 Heidelberg Germany D-69120
Citazione:
E. Oxelmark et al., "Mmf1p, a novel yeast mitochondrial protein conserved throughout evolution and involved in maintenance of the mitochondrial genome", MOL CELL B, 20(20), 2000, pp. 7784-7797

Abstract

A novel protein family (p14.5, or YERO57c/YJGFc) highly conserved throughout evolution has recently been identified. The biological role of these proteins is not yet well characterized. Two members of the p14.5 family are present in the yeast Saccharomyces cerevisiae. In this study, we have characterized some of the biological functions of the two yeast proteins. Mmf1p isa mitochondrial matrix factor, and homologous Mmf1p factor (Hmf1p) copurifies with the soluble cytoplasmic fraction. Delta mmf1 cells lose mitochondrial DNA (mtDNA) and have a decreased growth rate, while Delta hmf1 cells donot display any visible phenotype. Furthermore, we demonstrate by genetic analysis that Mmf1p does not play a direct role in replication and segregation of the mtDNA, rho(+) Delta mmf1 haploid cells can be obtained when tetrads are directly dissected on medium containing a nonfermentable carbon source. Our data also indicate that Mmf1p and Hmf1p have similar biological functions in different subcellular compartments. Hmf1p, when fused with the Mmf1p leader peptide, is transported into mitochondria and is able to functionally replace Mmf1p. Moreover, we show that homologous mammalian proteins are functionally related to Mmf1p. Human p14.5 localizes in yeast mitochondria and rescues the Delta mmf1-associated phenotypes. In addition, fractionation of rat liver mitochondria showed that rat p14.5, like Mmf1p, is a soluble protein of the matrix. Our study identifies a biological function for Mmf1p and furthermore indicates that this function is conserved between members of the p14.5 family.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 07:14:13