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Titolo:
Synaptic vesicle transporter expression regulates vesicle phenotype and quantal size
Autore:
Pothos, EN; Larsen, KE; Krantz, DE; Liu, Y; Haycock, JW; Setlik, W; Gershon, MD; Edwards, RH; Sulzer, D;
Indirizzi:
Columbia Univ, Dept Neurol, New York, NY 10032 USA Columbia Univ New YorkNY USA 10032 , Dept Neurol, New York, NY 10032 USA Columbia Univ, Dept Psychiat, New York, NY 10032 USA Columbia Univ New York NY USA 10032 Dept Psychiat, New York, NY 10032 USA New York State Psychiat Inst, Dept Neurosci, New York, NY 10032 USA New York State Psychiat Inst New York NY USA 10032 New York, NY 10032 USA Univ Calif San Francisco, Sch Med, Dept Neurol, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Univ Calif San Francisco, Sch Med, Dept Physiol, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Univ Pittsburgh, Sch Med, Dept Neurol, Pittsburgh, PA 15261 USA Univ Pittsburgh Pittsburgh PA USA 15261 Neurol, Pittsburgh, PA 15261 USA Univ Pittsburgh, Sch Med, Dept Neurosci, Pittsburgh, PA 15261 USA Univ Pittsburgh Pittsburgh PA USA 15261 eurosci, Pittsburgh, PA 15261 USA Columbia Univ, Dept Anat & Cell Biol, New York, NY 10032 USA Columbia Univ New York NY USA 10032 t & Cell Biol, New York, NY 10032 USA Louisiana State Univ, Med Ctr, Dept Biochem & Mol Biol, New Orleans, LA 70119 USA Louisiana State Univ New Orleans LA USA 70119 , New Orleans, LA 70119 USA
Titolo Testata:
JOURNAL OF NEUROSCIENCE
fascicolo: 19, volume: 20, anno: 2000,
pagine: 7297 - 7306
SICI:
0270-6474(20001001)20:19<7297:SVTERV>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
VESICULAR MONOAMINE TRANSPORTER-2; PHEOCHROMOCYTOMA PC12 CELLS; DOPAMINE NEURONS; IN-VITRO; NEUROTRANSMITTER TRANSPORT; TYROSINE-HYDROXYLASE; CHROMAFFIN GRANULES; GROWTH-FACTOR; ATT-20 CELLS; RAT-BRAIN;
Keywords:
VMAT2; monoamines; dopamine; quantal size; amperometry; vesicular transporter;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Sulzer, D Black Bldg,Room 305,650 W 168th St, New York, NY 10032 USA Black Bldg,Room 305,650 W 168th St New York NY USA 10032 32 USA
Citazione:
E.N. Pothos et al., "Synaptic vesicle transporter expression regulates vesicle phenotype and quantal size", J NEUROSC, 20(19), 2000, pp. 7297-7306

Abstract

While the transporters that accumulate classical neurotransmitters in synaptic vesicles have been identified, little is known about how their expression regulates synaptic transmission. We have used adenoviral-mediated transfection to increase expression of the brain vesicular monoamine transporterVMAT2 and presynaptic amperometric recordings to characterize the effects on quantal release. In presynaptic axonal varicosities of ventral midbrain neurons in postnatal culture, VMAT2 overexpression in small synaptic vesicles increased both quantal size and frequency, consistent with the recruitment of synaptic vesicles that do not normally release dopamine. This was confirmed using noncatecholaminergic AtT-20 cells, in which VMAT2 expression induced the quantal release of dopamine. The ability to increase quantal size in vesicles that were already competent for dopamine release was shown inPC12 cells, in which VMAT2 expression increased the quantal size but not the number of release events. These results demonstrate that vesicle transporters limit the rate of transmitter accumulation and can alter synaptic strength through two distinct mechanisms.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 20:16:10