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Titolo:
Studies on a mechanism by which cytosolic phospholipase A(2) regulates theexpression and function of type IIA secretory phospholipase A(2)
Autore:
Kuwata, H; Yamamoto, S; Miyazaki, Y; Shimbara, S; Nakatani, Y; Suzuki, H; Ueda, N; Yamamoto, S; Murakami, M; Kudo, I;
Indirizzi:
Showa Univ, Sch Pharmaceut Sci, Dept Hlth Chem, Shinagawa Ku, Tokyo 1428555, Japan Showa Univ Tokyo Japan 1428555 Chem, Shinagawa Ku, Tokyo 1428555, Japan Univ Tokushima, Sch Med, Dept Biochem, Tokushima 770, Japan Univ Tokushima Tokushima Japan 770 d, Dept Biochem, Tokushima 770, Japan
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 7, volume: 165, anno: 2000,
pagine: 4024 -
SICI:
0022-1767(20001001)165:7<4024:SOAMBW>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARACHIDONIC-ACID RELEASE; MURINE P388D(1) MACROPHAGES; LOW-DENSITY LIPOPROTEINS; PROSTAGLANDIN GENERATION; MESANGIAL CELLS; MAST-CELLS; CYCLOOXYGENASE-2; INDUCTION; FIBROBLASTS; RECEPTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Kudo, I Showa Univ, Sch Pharmaceut Sci, Dept Hlth Chem, Shinagawa Ku, 1-5-8 Hatanodai, Tokyo 1428555, Japan Showa Univ 1-5-8 Hatanodai Tokyo Japan 1428555 kyo 1428555, Japan
Citazione:
H. Kuwata et al., "Studies on a mechanism by which cytosolic phospholipase A(2) regulates theexpression and function of type IIA secretory phospholipase A(2)", J IMMUNOL, 165(7), 2000, pp. 4024

Abstract

Although it has been proposed that arachidonate release by several secretory phospholipase A(2) (sPLA(2)) isozymes is modulated by cytosolic PLA(2) (cPLA(2)), the cellular component(s) that intermediates between these two signaling PLA(2)s remains unknown. Here we provide evidence that 12- or 15-lipoxygenase (12/15-LOX), which lies downstream of cPLA(2), plays a pivotal role in cytokine-induced gene expression and function of sPLA(2)-IIA. The sPLA(2)-IIA expression and associated PGE(2) generation induced by cytokines in rat fibroblastic 3Y1 cells were markedly attenuated by antioxidants thatpossess 12/15-LOX inhibitory activity. 3Y1 cells expressed 12/15-LOX endogenously, and forcible overexpression of 12/15-LOX in these cells greatly enhanced cytokine-induced expression of sPLA(2)-IIA, with a concomitant increase in delayed PG generation, Moreover, studies using 293 cells stably transfected with sPLA(2)-IIA revealed that stimulus-dependent hydrolysis of membrane phospholipids by sPLA(2)-IIA was enhanced by overexpression of 12/15-LOX. These results indicate that the product(s) generated by the cPLA(2)-12/15-LOX pathway following cell activation may play two roles: enhancement of sPLA(2)-IIA gene expression and membrane sensitization that leads to accelerated sPLA(2)-IIA-mediated hydrolysis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 10:12:54