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Titolo:
Effects of a hexameric deoxyriboguanosine run conjugation into CpG oligodeoxynucleotides on their immunostimulatory potentials
Autore:
Lee, SW; Song, MK; Baek, KH; Park, YJ; Kim, JK; Lee, CH; Cheong, HK; Cheong, C; Sung, YC;
Indirizzi:
Pohang Univ Sci & Technol, Dept Life Sci, Ctr Biofunct Mol, Pohang 790784,South Korea Pohang Univ Sci & Technol Pohang South Korea 790784 g 790784,South Korea Korea Basic Sci Inst, Magnet Resonance Team, Taejon, South Korea Korea Basic Sci Inst Taejon South Korea nance Team, Taejon, South Korea
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 7, volume: 165, anno: 2000,
pagine: 3631 - 3639
SICI:
0022-1767(20001001)165:7<3631:EOAHDR>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
BACTERIAL-DNA; SCAVENGER RECEPTORS; DENDRITIC CELLS; IMMUNE ACTIVATION; INTERFERON-GAMMA; TH1 RESPONSES; CUTTING EDGE; MOTIFS; BINDING; MURINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Sung, YC Pohang Univ Sci & Technol, Dept Life Sci, Ctr Biofunct Mol, Pohang 790784,South Korea Pohang Univ Sci & Technol Pohang South Korea 790784 South Korea
Citazione:
S.W. Lee et al., "Effects of a hexameric deoxyriboguanosine run conjugation into CpG oligodeoxynucleotides on their immunostimulatory potentials", J IMMUNOL, 165(7), 2000, pp. 3631-3639

Abstract

CpG oligodeoxynucleotides (ODNs) are promising immunomodulatory agents fortreating human diseases and vaccine development. Phosphodiester CpG ODNs were demonstrated to have poor immunostimulatory potentials for cytokine production. However, the conjugation of consecutive deoxyriboguanosine residues, called a dG run, at the 3' terminus of phosphodiester CpG ODNs significantly enhanced TNF-alpha and IL-12 production from mouse splenic dendritic cells (DCs), The optimal induction of cytokine production was achieved by the addition of a hexameric dG (dG(6)) run, In contrast, the existence of a dG(6) run either at the 5' terminus of phosphodiester CpG ODNs or at the 3' terminus of phosphorothioate CpG ODNs diminished CpG-mediated cytokine induction, suggesting that the effects of a dG run depend on its location and the chemical property of the ODN backbone, respectively. In addition, we provided the evidence that the conjugation of a dG(6) run caused the structural transformation of CpG ODNs, which facilitates their targeting into mouse APCs such as splenic DCs, B cells, and peritoneal macrophages with a scavenger receptor type A ligand specificity. Among primary APCs, DCs were the most potent for CpG ODN-mediated IL-12 production. Furthermore, we demonstrated that the conjugation of a dG(6) run into the 3' terminus of phosphodiester CpG ODNs was crucial for their ability to generate Th1 immunity in vivo. Thus, the conjugation of a dG(6) run into phosphodiester CpG ODNs would bean alternative way to optimize their immunostimulatory potentials in vitroand in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 03:36:51