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Titolo:
Thrombin induces NO release from cultured rat microglia via protein kinaseC, mitogen-activated protein kinase, and NF-kappa B
Autore:
Ryu, J; Pyo, H; Jou, I; Joe, EH;
Indirizzi:
Ajou Univ, Sch Med, Dept Pharmacol, Suwon 442721, South Korea Ajou Univ Suwon South Korea 442721 Pharmacol, Suwon 442721, South Korea
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 39, volume: 275, anno: 2000,
pagine: 29955 - 29959
SICI:
0021-9258(20000929)275:39<29955:TINRFC>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-EXPRESSION; ENDOTHELIAL-CELLS; TYROSINE KINASE; GLIOMA-CELLS; RECEPTOR; BRAIN; LIPOPOLYSACCHARIDE; ASTROCYTES; PATHWAY; INDUCTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Joe, EH San-5 Woncheon-dong, Suwon 442721, Kyunggido, South Korea San-5 Woncheon-dong Suwon Kyunggido South Korea 442721 outh Korea
Citazione:
J. Ryu et al., "Thrombin induces NO release from cultured rat microglia via protein kinaseC, mitogen-activated protein kinase, and NF-kappa B", J BIOL CHEM, 275(39), 2000, pp. 29955-29959

Abstract

Microglia, brain resident macrophages, become activated in brains injured due to trauma, ischemia, or neurodegenerative diseases. In this study, we found that thrombin treatment of microglia induced NO release/ inducible nitric-oxide synthase expression, a prominent marker of activation. The effectof thrombin on NO release increased dose-dependently within the range of 5-20 units/ml. In immunoblot analyses, inducible nitric-oxide synthase expression was detected within 9 h after thrombin treatment. This effect of thrombin was significantly reduced by protein kinase C inhibitors, such as Go6976, bisindolylmaleimide, and Ro31-8220. Within 15 min, thrombin activated three subtypes of mitogen-activated protein kinases: extracellular signal-regulated kinase, p38, and c-Jun N-terminal kinase/stress-activated protein kinase. Inhibition of the extracellular signal-regulated kinase pathway and p38 reduced the NO release of thrombin-treated microglia. Thrombin also activated nuclear factor kappa B (NF-kappa KB) within 5 min, and N-acetyl cysteine, an inhibitor of NF-kappa B, reduced NO release. However, thrombin receptor agonist peptide (an agonist of protease activated receptor-1 (PAR-1)), could not mimic the effect of thrombin, and cathepsin G, a PAR 1 inhibitor, did not reduce the effect of thrombin. These results suggest that thrombin can activate microglia via protein kinase C, mitogen-activated protein kinases, and NF-kappa B but that this occurs independently of PAR-1.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 01:27:27