Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Single cell Ras-GTP analysis reveals altered Ras activity in a subpopulation of neurofibroma Schwann cells but not fibroblasts
Autore:
Sherman, LS; Atit, R; Rosenbaum, T; Cox, AD; Ratner, N;
Indirizzi:
Univ Cincinnati, Coll Med, Dept Cell Biol Neurobiol & Anat, Cincinnati, OH45267 USA Univ Cincinnati Cincinnati OH USA 45267 l & Anat, Cincinnati, OH45267 USA Univ Dusseldorf, Childrens Hosp, Dept Neuropediat, D-4000 Dusseldorf, Germany Univ Dusseldorf Dusseldorf Germany D-4000 at, D-4000 Dusseldorf, Germany Univ N Carolina, Dept Radiat Oncol, Chapel Hill, NC 27599 USA Univ N Carolina Chapel Hill NC USA 27599 Oncol, Chapel Hill, NC 27599 USA Univ N Carolina, Dept Pharmacol, Chapel Hill, NC 27599 USA Univ N Carolina Chapel Hill NC USA 27599 macol, Chapel Hill, NC 27599 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 39, volume: 275, anno: 2000,
pagine: 30740 - 30745
SICI:
0021-9258(20000929)275:39<30740:SCRARA>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
MALIGNANT MYELOID DISORDERS; TYPE-1 GENE; NF1 GENE; SIGNALING PATHWAY; BINDING DOMAIN; DROSOPHILA NF1; PROTEIN-KINASE; BONE-MARROW; ACTIVATION; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Ratner, N Univ Cincinnati, Coll Med, Dept Cell Biol Neurobiol & Anat, 3125Eden Ave,Cincinnati, OH 45267 USA Univ Cincinnati 3125 Eden Ave CincinnatiOH USA 45267 45267 USA
Citazione:
L.S. Sherman et al., "Single cell Ras-GTP analysis reveals altered Ras activity in a subpopulation of neurofibroma Schwann cells but not fibroblasts", J BIOL CHEM, 275(39), 2000, pp. 30740-30745

Abstract

Neurofibromatosis type 1 (NF1) is a common genetic disorder characterized by multiple neurofibromas, peripheral nerve tumors containing mainly Schwann cells and fibroblasts. The NF1 gene encodes neurofibromin, a tumor suppressor postulated to function in part as a Ras GTPase-activating protein. Theroles of different cell types and of elevated Ras-GTP in neurofibroma formation are unclear. To determine which neurofibroma cell type has altered Ras-GTP regulation, we developed an immunocytochemical assay for active, GTP-bound Ras. In NIH 3T3 cells, the assay detected overexpressed, constitutively activated K-, N-, and Ha-Ras and insulin-induced endogenous Ras GTP. In dissociated neurofibroma cells from NF1 patients, Ras-GTP was elevated in Schwann cells but not fibroblasts. Twelve to 62% of tumor Schwann cells showed elevated Ras-GTP, unexpectedly revealing neurofibroma Schwann cell heterogeneity. Increased basal Ras-GTP did not correlate with increased cell proliferation. Normal human Schwann cells, however, did not demonstrate elevated basal Ras activity. Furthermore, compared with cells from wild type littermates, Ras-GTP was elevated in all mouse Nf1(-/-) Schwann cells but neverin Nf1(-/-) mouse fibroblasts. Our results indicate that Ras activity is detectably increased in only some neurofibroma Schwann cells and suggest that neurofibromin is not an essential regulator of Ras activity in fibroblasts.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 20:25:07