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Titolo:
PARTHENOGENETIC ACTIVATION OF MOUSE EGGS BY MICROINJECTION OF A TRUNCATED C-KIT TYROSINE KINASE PRESENT IN SPERMATOZOA
Autore:
SETTE C; BEVILACQUA A; BIANCHINI A; MANGIA F; GEREMIA R; ROSSI P;
Indirizzi:
UNIV ROMA TOR VERGATA,DIPARTIMENTO SANITA PUBBL & BIOL CELLULARE,SEZIONE ANAT,VIA O RAIMONDO 8 I-00173 ROME ITALY UNIV ROMA TOR VERGATA,DIPARTIMENTO SANITA PUBBL & BIOL CELLULARE,SEZIONE ANAT I-00173 ROME ITALY UNIV ROMA LA SAPIENZA,IST ISTOL EMBRIOL GEN ROME ITALY UNIV ROMA LA SAPIENZA,DIPARTIMENTO PSICOL ROME ITALY
Titolo Testata:
Development
fascicolo: 11, volume: 124, anno: 1997,
pagine: 2267 - 2274
SICI:
0950-1991(1997)124:11<2267:PAOMEB>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR RECEPTORS; MAP KINASE; MEIOTIC MATURATION; PROTEIN-KINASE; IN-VITRO; OOCYTES; FERTILIZATION; EXPRESSION; SPERM; CELLS;
Keywords:
C-KIT; SPERMATOZOA; METAPHASE II ARREST; EGG ACTIVATION; CALCIUM; TYROSINE-KINASE; TRUNCATED-RECEPTOR; MAP KINASE; PHOSPHOLIPASE C;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
C. Sette et al., "PARTHENOGENETIC ACTIVATION OF MOUSE EGGS BY MICROINJECTION OF A TRUNCATED C-KIT TYROSINE KINASE PRESENT IN SPERMATOZOA", Development, 124(11), 1997, pp. 2267-2274

Abstract

A truncated form of the c-kit tyrosine kinase receptor, correspondingto the phosphotransferase portion of the cytoplasmic catalytic domainand the carboxyterminus (tr-kit), is accumulated during late mouse spermiogenesis, Here we report that tr-kit is specifically localized in the residual sperm cytoplasm, with maximal accumulation in the midpiece of the flagellum, suggesting that it can enter the egg during fertilization, Microinjection of extracts from COS cells expressing a recombinant tr-kit protein into metaphase II-arrested mouse oocytes caused complete oocyte activation, including cortical granule exocytosis, completion of the 2(nd) meiotic division, formation of a parthenogenetic pronucleus and progression through cleavage stages, No activation abovebackground levels was obtained with extracts from mock-transfected COS cells. Similar results were obtained by microinjection of in vitro synthesized tr-kit mRNA into metaphase II-arrested oocytes, Tr-kit-induced parthenogenetic egg activation was completely inhibited by oocyte preincubation with the Ca2+-chelating agent BAPTA-AM or with a specific inhibitor of phospholipase C activity, Tr-kit-induced egg activationwas associated with a decrease in activity of mitogen-activated protein kinase, an essential component of the cytostatic factor, These results candidate tr-kit as a putative sperm factor required for triggering activation of mouse eggs at fertilization.

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Documento generato il 27/11/20 alle ore 22:19:34