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Titolo:
IMMUNOMAGNETIC SELECTION OF CD34(-BLOOD STEM-CELLS FOR AUTOGRAFTING IN PATIENTS WITH BREAST-CANCER() PERIPHERAL)
Autore:
HOHAUS S; PFORSICH M; MUREA S; ABDALLAH A; LIN YS; FUNK L; VOSO MT; KAUL S; SCHMID H; WALLWIENER D; HAAS R;
Indirizzi:
UNIV HEIDELBERG,DEPT INTERNAL MED 5,HOSP STR 3 D-69115 HEIDELBERG GERMANY UNIV HEIDELBERG,DEPT INTERNAL MED 5 D-69115 HEIDELBERG GERMANY UNIV HEIDELBERG,DEPT OBSTET & GYNAECOL HEIDELBERG GERMANY GERMAN CANC RES CTR,CLIN COOPERAT UNIT D-6900 HEIDELBERG GERMANY
Titolo Testata:
British Journal of Haematology
fascicolo: 4, volume: 97, anno: 1997,
pagine: 881 - 888
SICI:
0007-1048(1997)97:4<881:ISOCSF>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
HIGH-DOSE CHEMOTHERAPY; BONE-MARROW TRANSPLANTATION; COLONY-STIMULATING FACTOR; PROGENITOR CELLS; ENGRAFTMENT; LYMPHOMA; RELAPSE; THERAPY; RISK; CSF;
Keywords:
POSITIVE SELECTION; CD34(+) CELLS; AUTOLOGOUS TRANSPLANTATION; BREAST CANCER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
22
Recensione:
Indirizzi per estratti:
Citazione:
S. Hohaus et al., "IMMUNOMAGNETIC SELECTION OF CD34(-BLOOD STEM-CELLS FOR AUTOGRAFTING IN PATIENTS WITH BREAST-CANCER() PERIPHERAL)", British Journal of Haematology, 97(4), 1997, pp. 881-888

Abstract

Contamination of transplants with tumour cells may contribute to relapse after peripheral blood stem cell transplantation (PBSCT). We studied the feasibility of CD34(+) cell selection from blood-derived autografts obtained following G-CSF-supported cytotoxic chemotherapy in a group of 25 patients with breast cancer (10 with high-risk stage II/III and 15 with stage IV without bone or bone marrow involvement). Using immunomagnetic beads (Isolex 300 SA, Baxter) CD34(+) cells were enriched and released by chymopapain resulting in a median purity of 95% (range 82-99%) and a median recovery of 80% (range 27-132%), The enrichment procedure did not change the proportion of CD34(+) subsets coexpressing HLA-DR, CD38 and Thy-1, while L-selectin was removed from the cellsurface following selection. Using a sensitive immunocytological technique with a cocktail of epithelial-specific antibodies (anti-cytokeratin 8, 18 and 19; HEA125; BM7 and BM8), we leukaphereses products contained epithelial cells, whereas the selected CD34(+) cell fraction wasfree of tumour cells. A neutrophil count of 0.5 x 10(9)/l and a platelet count of 20 x10(9)/l was reached after a median time of 14 and 10dfollowing 40 high-dose chemotherapy (HDC) cycles, Our results indicate that immunomagnetic selection of CD34(+) cells yields highly purified autografts devoid of tumour cells whereas the engraftment ability ofthe progenitor and stem cells is fully retained.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 21:00:26