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Titolo:
Apoptosis and cell proliferation in the metaplasia-dysplasia-carcinoma-sequence of Barrett's esophagus
Autore:
Halm, U; Tannapfel, A; Breitung, B; Breidert, M; Wittekind, CW; Mossner, J;
Indirizzi:
Univ Leipzig, Dept Internal Med 2, D-04103 Leipzig, Germany Univ Leipzig Leipzig Germany D-04103 nal Med 2, D-04103 Leipzig, Germany Univ Leipzig, Inst Pathol, D-04103 Leipzig, Germany Univ Leipzig LeipzigGermany D-04103 st Pathol, D-04103 Leipzig, Germany Tech Univ Dresden, Dept Internal Med 1, D-8027 Dresden, Germany Tech Univ Dresden Dresden Germany D-8027 Med 1, D-8027 Dresden, Germany
Titolo Testata:
HEPATO-GASTROENTEROLOGY
fascicolo: 34, volume: 47, anno: 2000,
pagine: 962 - 966
SICI:
0172-6390(200007/08)47:34<962:AACPIT>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
P53 PROTEIN ACCUMULATION; NUCLEAR ANTIGEN; GROWTH-FACTOR; ADENOCARCINOMA; DEATH; DISEASE; PROGRESSION; EXPRESSION; MARKERS; KI-67;
Keywords:
apoptosis; Barrett's esophagus; adenocarcinoma; p53; Ki67 antibody;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Halm, U Univ Leipzig, Dept Internal Med 2, Philipp Rosenthal Str 27, D-04103 Leipzig, Germany Univ Leipzig Philipp Rosenthal Str 27 Leipzig Germany D-04103 any
Citazione:
U. Halm et al., "Apoptosis and cell proliferation in the metaplasia-dysplasia-carcinoma-sequence of Barrett's esophagus", HEP-GASTRO, 47(34), 2000, pp. 962-966

Abstract

Background/Aims: The regulation of apoptosis as a distinctive form of celldeath and proliferation in the process of carcinogenesis in Barrett's esophagus is poorly understood. To investigate regulation of apoptosis, proliferation and the participation of the tumor suppressor gene p53, we examined these parameters in Barrett's metaplasia, dysplasia, and adenocarcinoma. Methodology: Apoptotic cells were identified and quantified in tissue specimens of 45 patients with different stages of Barrett's esophagus and normal fundus epithelium, respectively, using the in situ end-labeling and electron microscopy method in combination with morphological criteria. The tumorsuppressor gene p53 was examined by direct sequencing of exon 4-8 as well as immunohistochemically. The proliferative activity was assessed by Ki67 immunostaining. Results: Apoptotic cell death, identified by the in situ end-labeling and ultrastructural technique was significantly increased in Barrett's epithelium with intestinal metaplasia than in specimens with normal fundic epithelium and Barrett's carcinomas (P<0.01). The proliferative activity, defined as Ki67 labeling index, was highest in adenocarcinomas (P<0.01). P53 mutations were found in 8/9 adeno carcinomas and 2/5 specimens with dysplasia. Apoptosis was lower in p53 positive specimens of the metaplasia-dysplasia-carcinoma-sequence than in p53 negative specimens of Bal rett's esophagus (P<0.05). Conclusions: The higher levels of both apoptosis and proliferation indicate an increased cell turnover in Barrett's epithelium. Apoptosis seems to maintain tissue homeostasis, which is regulated by p53, and gradually lost inthe metaplasia-dysplasia-carcinoma-sequence of Barrett's esophagus.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/21 alle ore 16:09:08