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Titolo:
Quantification of mitochondrial DNA in human blood cells using an automated detection system
Autore:
Meissner, C; Mohamed, SA; Klueter, H; Hamann, K; von Wurmb, N; Oehmichen, M;
Indirizzi:
Med Univ Lubeck, Dept Legal Med, D-23562 Lubeck, Germany Med Univ Lubeck Lubeck Germany D-23562 egal Med, D-23562 Lubeck, Germany Med Univ Lubeck, Inst Immunol & Transfus Med, D-23562 Lubeck, Germany Med Univ Lubeck Lubeck Germany D-23562 sfus Med, D-23562 Lubeck, Germany PE Biosyst, Weiterstadt, Germany PE Biosyst Weiterstadt GermanyPE Biosyst, Weiterstadt, Germany Univ Calif Davis, VM Mol Biosci, Davis, CA 95616 USA Univ Calif Davis Davis CA USA 95616 s, VM Mol Biosci, Davis, CA 95616 USA
Titolo Testata:
FORENSIC SCIENCE INTERNATIONAL
fascicolo: 1-3, volume: 113, anno: 2000,
pagine: 109 - 112
SICI:
0379-0738(20000911)113:1-3<109:QOMDIH>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
DELETION; PLATELETS; TISSUES;
Keywords:
mitochondrial DNA; deletion; real-time PCR; blood cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
9
Recensione:
Indirizzi per estratti:
Indirizzo: Meissner, C Med Univ Lubeck, Dept Legal Med, Kahlhorststr 31-35, D-23562 Lubeck, Germany Med Univ Lubeck Kahlhorststr 31-35 Lubeck Germany D-23562 any
Citazione:
C. Meissner et al., "Quantification of mitochondrial DNA in human blood cells using an automated detection system", FOREN SCI I, 113(1-3), 2000, pp. 109-112

Abstract

The 4977 bp deletion of mitochondrial DNA (mtDNA) accumulates in postmitotic tissues with advancing age. The purpose of our study was to detect and quantify these deletion even in blood cells with a high turnover activity. Whole venous blood, isolated human platelets and peripheral blood mononuclear cells (PBMCs) were collected from 10 unrelated donors aged 20-71 years and total DNA was extracted. PCR was performed for total and mutated mtDNA using two different primer pairs and two fluorogenic probes labeled with the fluorescent dyes FAM and VIC. Specific PCR products were generated, detected and quantified in a real-time PCR. The amplification products of total and deleted mtDNA could be detected in each sample and did not exhibit any differences in the amount of the deleted mtDNA in whole blood, human platelets or PBMCs. Our data did not show any accumulation of the 4977 bp deletion with increasing age as it was observed for several other tissues. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 06:33:34