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Titolo:
Ca2+ movement from leaky sarcoplasmic reticulum during contraction of rat arterial smooth muscles
Autore:
Asano, M; Nomura, Y;
Indirizzi:
Nagoya City Univ, Sch Med, Dept Pharm, Mizuho Ku, Nagoya, Aichi 4678601, Japan Nagoya City Univ Nagoya Aichi Japan 4678601 Nagoya, Aichi 4678601, Japan
Titolo Testata:
EUROPEAN JOURNAL OF PHARMACOLOGY
fascicolo: 3, volume: 404, anno: 2000,
pagine: 327 - 339
SICI:
0014-2999(20000922)404:3<327:CMFLSR>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPONTANEOUSLY HYPERTENSIVE RATS; POTENT VASOCONSTRICTOR ACTIONS; SUPERFICIAL BUFFER BARRIER; LIGHT CHAIN KINASE; CYCLOPIAZONIC ACID; POSSIBLE MECHANISM; FEMORAL ARTERIES; PROTEIN-KINASE; RYANODINE; CALCIUM;
Keywords:
sarcoplasmic reticulum; superficial buffer barrier hypothesis; Ca2+-induced Ca2+ release channels; Ca2+ movement; Ca2+ influx; Ca2+ channel, L-type;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Asano, M Nagoya City Univ, Sch Med, Dept Pharm, Mizuho Ku, Mizuho Cho, Nagoya, Aichi 4678601, Japan Nagoya City Univ Mizuho Cho Nagoya Aichi Japan 4678601 601, Japan
Citazione:
M. Asano e Y. Nomura, "Ca2+ movement from leaky sarcoplasmic reticulum during contraction of rat arterial smooth muscles", EUR J PHARM, 404(3), 2000, pp. 327-339

Abstract

To examine the Ca2+ buffering function of the sarcoplasmic reticulum during arterial contraction, we studied Ca2+ movement during stimulation with Kor norepinephrine in arteries with a leaky sarcoplasmic reticulum. Responses were compared in endothelium-denuded strips of femoral, mesenteric and carotid arteries of the rat. To make the sarcoplasmic reticulum leaky to Ca2, Ca2+-induced Ca2+ release channels of the sarcoplasmic reticulum were locked open by treatment with ryanodine plus caffeine. After ryanodine treatment, the contractile responses to K+ (3-20 mM) were augmented when comparedwith control responses in femoral and mesenteric arteries, but were inhibited in the carotid artery. Similar results were obtained when the contractile responses to norepinephrine were determined. The inhibition by ryanodineof the K+- or norepinephrine-contractions seen in the carotid artery was reversed by pretreatment with cyclopiazonic acid (10 mu M), an inhibitor of the sarcoplasmic reticulum Ca2+-ATPase, but was not by charybdotoxin (100 nM), a blocker of Ca2+-activated K+ channels. We conclude that (1) after ryanodine treatment, Ca2+ entering from the extracellular space during stimulation with K+ or norepinephrine is first taken up into the leaky sarcoplasmic reticulum and then reaches the myofilaments in femoral and mesenteric arteries, while in the carotid artery, C2+ leaked from the sarcoplasmic reticulum reaches mainly the plasma membrane from where it is extruded into the extracellular space, and (2) the different movement of Ca2+ may be due to the relative location of the sarcoplasmic reticulum in the smooth muscle cellof each artery. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 10:29:35