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Titolo:
Prolongation of murine hybridoma cell survival in stationary batch cultureby Bcl-xL expression
Autore:
Charbonneau, JR; Gauthier, ER;
Indirizzi:
Laurentian Univ, Dept Chem & Biochem, Cellular Biochem Res Lab, Sudbury, ON P3E 2C6, Canada Laurentian Univ Sudbury ON Canada P3E 2C6 ab, Sudbury, ON P3E 2C6, Canada
Titolo Testata:
CYTOTECHNOLOGY
fascicolo: 1-2, volume: 34, anno: 2000,
pagine: 131 - 139
SICI:
0920-9069(200010)34:1-2<131:POMHCS>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
MYELOMA CELLS; APOPTOTIC DEATH; PROTEIN-FREE; GENE; OVEREXPRESSION; BAX; REGULATORS; BCL-X(L); SYSTEMS; FAMILY;
Keywords:
apoptosis; bcl-xL; cell growth; cell viability; hybridoma; myeloma;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Gauthier, ER Laurentian Univ, Dept Chem & Biochem, Cellular Biochem Res Lab, 935 RamseyLake Rd, Sudbury, ON P3E 2C6, Canada Laurentian Univ 935 Ramsey Lake Rd Sudbury ON Canada P3E 2C6
Citazione:
J.R. Charbonneau e E.R. Gauthier, "Prolongation of murine hybridoma cell survival in stationary batch cultureby Bcl-xL expression", CYTOTECHNOL, 34(1-2), 2000, pp. 131-139

Abstract

While the ectopic expression of the anti-apoptotic protein Bcl-2 has been shown to significantly increase both cell viability and antibody productionin batch culture, some cell lines are refractory to these manipulations. For example, the NS/O and the P3x63Ag8.653 murine myelomas, which express high endogenous levels of the Bcl-2 homologue Bcl-xL, are both resistant to the anti-apoptotic effect of Bcl-2. This indicates that, in these cells, Bcl-2 and Bcl-xL may be functionally redundant. In order to define the role which Bcl-xL plays in hybridoma cultures, we used the Sp2/0-Ag14 cell line. This murine hybridoma expresses low levels of Bcl-xL and is highly sensitiveto apoptosis induction by cycloheximide (CHX) and by amino acid depletion. Bcl-xL-transfected Sp2/0-Ag14 cells were more resistant than the wild typeand the plasmid-containing cells to apoptosis induced by CHX and by glutamine depletion. Moreover, when compared to the vector-transfected control, Bcl-xL-Sp2/0 cells exhibited a substantial increase in viability in stationary batch culture. Interestingly, Sp2/0-Ag14 cells overexpressing Bcl-xL showed a growth behaviour that was similar to the parent myeloma cell line P3x63Ag8.653. Our results suggest that Bcl-xL expression levels are sufficientto account for the relative robustness of some hybridoma cell lines in stationary batch cultures.

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Documento generato il 24/11/20 alle ore 10:55:56