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Titolo:
Altered expression of neurofilament subunits in diisopropyl phosphorofluoridate-treated hen spinal cord and their presence in axonal aggregations
Autore:
Gupta, RP; Abdel-Rahman, A; Jensen, KF; Abou-Donia, MB;
Indirizzi:
Duke Univ, Med Ctr, Dept Pharmacol & Canc Biol, Durham, NC 27706 USA Duke Univ Durham NC USA 27706 Pharmacol & Canc Biol, Durham, NC 27706 USA US EPA, Div Neurotoxicol, Res Triangle Pk, NC 27711 USA US EPA Res Triangle Pk NC USA 27711 oxicol, Res Triangle Pk, NC 27711 USA
Titolo Testata:
BRAIN RESEARCH
fascicolo: 1-2, volume: 878, anno: 2000,
pagine: 32 - 47
SICI:
0006-8993(20000929)878:1-2<32:AEONSI>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERMEDIATE FILAMENT PROTEINS; INDUCED DELAYED NEUROTOXICITY; NF-H; CYTOSKELETAL PROTEINS; (DFP)-TREATED HEN; MOTOR-NEURONS; RADIAL GROWTH; PHOSPHORYLATION; BINDING; DISEASE;
Keywords:
neurofilament proteins; diisopropyl phosphorofluoridate; chicken; spinal cord; neurotoxicity; organophosphorus ester-induced delayed neuropathy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Abou-Donia, MB Duke Univ, Med Ctr, Dept Pharmacol & Canc Biol, POB 3813, Durham, NC 27706USA Duke Univ POB 3813 Durham NC USA 27706 Durham, NC 27706USA
Citazione:
R.P. Gupta et al., "Altered expression of neurofilament subunits in diisopropyl phosphorofluoridate-treated hen spinal cord and their presence in axonal aggregations", BRAIN RES, 878(1-2), 2000, pp. 32-47

Abstract

Diisopropyl phosphorofluoridate (DFP) is an organophosphorus ester, which produces organophosphorus ester-induced delayed neuropathy (OPIDN) in hen and other sensitive species. A single dose of DFP (1.7 mg/kg, sc.) produces mild ataxia in 7-14 days in hens, which develops into severe ataxia or paralysis with the progression of disease. OPIDN is associated with axonal swellings and degeneration of axons. This study was carried out to investigate the expression of neurofilament (NF) subunits in the spinal cord of DFP-treated hens. Hens were treated with a single dose of DFP and sacrificed 1, 5,10, and 20 days post-treatment. Western blot analysis showed increased expression of middle molecular weight neurofilament protein (NF-M), and decreased expression of high molecular weight (NF-H) and low molecular weight (NF-L) neurofilament proteins in the 2 M urea extracts of spinal cord particulate fraction. These changes were observed within 24 h of DFP administrationand persisted for 10-20 days. Thus, there was increase in the stoichiometry of NF-M:NF-L in the spinal cord of DFP-treated hens. Immunoprecipitation,cross-linking, and two-dimensional polyacrylamide gel electrophoresis showed the presence of heterodimers, but not heterotetramers, in the hen spinalcord extract. Immunohistochemical staining revealed the presence of all three NF subunits in the cytoskeletal inclusions in DFP-treated hen spinal cord cross-sections. The results suggested that each NF subunit might be accumulated by a different mechanism in the axonal aggregations of DFP-treated hen. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 14:20:05