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Titolo:
Preparation and analysis of fetal liver extracts
Autore:
Zwicky, C; Gerber, S; Gasparini, D; Forestier, F; Hohlfeld, P; Tissot, JD; Schneider, P;
Indirizzi:
Serv Reg Vaudois Sanguine, CH-1005 Lausanne, Switzerland Serv Reg Vaudois Sanguine Lausanne Switzerland CH-1005 anne, Switzerland CHU Vaudois, Dept Obstet & Gynecol, CH-1011 Lausanne, Switzerland CHU Vaudois Lausanne Switzerland CH-1011 , CH-1011 Lausanne, Switzerland Inst Puericulture, Serv Med & Biol Foetales, Paris, France Inst Puericulture Paris France Serv Med & Biol Foetales, Paris, France
Titolo Testata:
BONE MARROW TRANSPLANTATION
fascicolo: 6, volume: 26, anno: 2000,
pagine: 667 - 671
SICI:
0268-3369(200009)26:6<667:PAAOFL>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC STEM-CELLS; ENDOTHELIAL-CELLS; TRANSPLANTATION; EXPRESSION; TOLERANCE; CD34; MICE; INDUCTION; DISEASES;
Keywords:
fetal stem cells; hematopoietic stem cells; in utero transplantation; CD34(+) cells; enndothelial cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Schneider, P Serv Reg Vaudois Sanguine, Rue Bugnon 27, CH-1005 Lausanne, Switzerland Serv Reg Vaudois Sanguine Rue Bugnon 27 Lausanne Switzerland CH-1005
Citazione:
C. Zwicky et al., "Preparation and analysis of fetal liver extracts", BONE MAR TR, 26(6), 2000, pp. 667-671

Abstract

The aim of this work is to describe the techniques that have been used forpreparation and analysis of whole fetal liver extracts destined for in utero transplantation. Nine fetal livers between 12 and 17 weeks of gestation were prepared: cell counts and assessment of the hematopoietic cell viability were performed on cell suspensions. Hepatocytes represented 40 to 80% ofthe whole cell population. The remaining cells were constituted by hematopoietic cells (mainly erythroblasts), as well as by endothelial cells, The latter expressed CD34 on their surface, interfering with the assessment of CD34(+) hematopoietic cells by flow cytometry, Direct visual morphologic control using alkaline phosphatase anti-alkaline phosphatase techniques was needed to differentiate hematopoietic from extra-hematopoietic CD34(+) cells. Between 3.0 and 34.6 x 10(6) CD34(+) viable hematopoietic cells were collected per fetal liver, Adequate differentiation of these cells into burst-forming units erythroid (BFU-E), colony-forming units granulocyte-macrophage (CFU-GM), and colony-forming units granulocyte erythroid macrophage megakaryocyte (CFU-GEMM) has been shown for each sample in clonogeneic cultures. In conclusion, fetal liver is a potential source of hematopoietic stem cells. Their numeration, based on the presence of CD34, is hampered by the expression of this antigen on other cells contained in the liver cell extract, in particular endothelial cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 22:13:22