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Titolo:
Homing and engraftment potential of Sca-1(+)lin(-) cells fractionated on the basis of adhesion molecule expression and position in cell cycle
Autore:
Orschell-Traycoff, CM; Hiatt, K; Dagher, RN; Rice, S; Yoder, MC; Srour, EF;
Indirizzi:
Indiana Univ, Sch Med, Div Hematol Oncol, Indianapolis, IN 46202 USA Indiana Univ Indianapolis IN USA 46202 Oncol, Indianapolis, IN 46202 USA Indiana Univ, Sch Med, Dept Med, Indiana Elks Canc Res Ctr, Indianapolis, IN 46202 USA Indiana Univ Indianapolis IN USA 46202 es Ctr, Indianapolis, IN 46202 USA Indiana Univ, Sch Med, Dept Pediat, Herman B Wells Ctr Pediat Res, Indianapolis, IN 46202 USA Indiana Univ Indianapolis IN USA 46202 at Res, Indianapolis, IN 46202 USA Indiana Univ, Sch Med, Dept Microbiol & Immunol, Indianapolis, IN 46202 USA Indiana Univ Indianapolis IN USA 46202 mmunol, Indianapolis, IN 46202 USA
Titolo Testata:
BLOOD
fascicolo: 4, volume: 96, anno: 2000,
pagine: 1380 - 1387
SICI:
0006-4971(20000815)96:4<1380:HAEPOS>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC PROGENITOR CELLS; EX-VIVO EXPANSION; BONE-MARROW; STEM-CELLS; PERIPHERAL-BLOOD; CD34(+) CELLS; IN-VITRO; INTEGRIN ALPHA-4-BETA-1; PLATELET RECOVERY; MURINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Orschell-Traycoff, CM Indiana Univ, Sch Med, Div Hematol Oncol, Indianapolis, IN 46202 USA Indiana Univ Indianapolis IN USA 46202 IN 46202 USA
Citazione:
C.M. Orschell-Traycoff et al., "Homing and engraftment potential of Sca-1(+)lin(-) cells fractionated on the basis of adhesion molecule expression and position in cell cycle", BLOOD, 96(4), 2000, pp. 1380-1387

Abstract

Engraftment potential of hematopoietic stem cells (HSCs) is likely to be dependent an several factors including expression of certain adhesion molecules (AMs) and degree of mitotic quiescence. The authors investigated the functional properties and engraftment potential of Sca-1(+)lin(-) cells subfractianated on the basis of expression, or lack thereof, of CD11a, CD43, CD49d, CD49e, or CD62L and correlated that expression with cell cycle status and proliferative potential of engrafting fractions. Donor-derived chimerismin mice receiving CD49e(+) or CD43(+) Sca-1(+)lin(-) cells was greater than that in mice receiving cells lacking these 2 markers, while Sca-1(+)lin(-) cells positive for CD11a and CD62L and bright for CD49d expression mediated minimal engraftment AM phenotypes enriched for engraftment potential contained the majority of high proliferative potential-colony forming cells, low proliferative potential-colony forming cells, and cells providing rapid in vitro expansion, Cell cycle analysis of AM subpopulations revealed that,regardless of their bone marrow repopulating potential, Sca-1(+)lin(-) AM(-) cells contained a higher percentage of cells in G(0)/G(1) than their AM() counterparts. Interestingly, engrafting phenotypes, regardless of the status of their AM expression, were quicker to exit G(0)/G(1) following in vitro cytokine stimulation than their opposing phenotypes, When engrafting phenotypes of Sca-1(+)lin(-) AM(+) or AM(-) cells were further fractionated by Hoechst 33342 into G(0)/G(1) or S/G(2)+M, cells providing long-term engraftment were predominantly contained within the quiescent fraction. These results define a theoretical phenotype of a Sca-1(+)lin(-) engrafting cell asone that is mitotically quiescent, CD43(+), CD49e(+), CD11a(-), CD49d(dlm), and CD62L(-). Furthermore, these data suggest that kinetics of in vitro proliferation may be a good predictor of engraftment potential of candidate populations of HSCs. (Blood, 2000;96: 1380-1387) (C) 2000 by The American Society of Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 18:43:08