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Titolo:
Highly efficient gene transfer into cord blood nonobese diabetic/severe combined immunodeficiency repopulating cells by oncoretroviral vector particles pseudotyped with the feline endogenous retrovirus (RD114) envelope protein
Autore:
Kelly, PF; Vandergriff, J; Nathwani, A; Nienhuis, AW; Vanin, EF;
Indirizzi:
St Jude Childrens Res Hosp, Div Expt Hematol, Dept Hematol Oncol, Memphis,TN 38105 USA St Jude Childrens Res Hosp Memphis TN USA 38105 col, Memphis,TN 38105 USA
Titolo Testata:
BLOOD
fascicolo: 4, volume: 96, anno: 2000,
pagine: 1206 - 1214
SICI:
0006-4971(20000815)96:4<1206:HEGTIC>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
APE LEUKEMIA-VIRUS; VESICULAR STOMATITIS-VIRUS; HUMAN HEMATOPOIETIC-CELLS; GREEN FLUORESCENT PROTEIN; AMINO-ACID TRANSPORTER; LONG-TERM ENGRAFTMENT; HIGH-LEVEL EXPRESSION; EX-VIVO CULTURE; IN-VIVO; BONE-MARROW;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Kelly, PF St Jude Childrens Res Hosp, Div Expt Hematol, Dept Hematol Oncol, 332 N Lauderdale St,Room D-4026, Memphis, TN 38105 USA St Jude Childrens Res Hosp 332 N Lauderdale St,Room D-4026 Memphis TN USA 38105
Citazione:
P.F. Kelly et al., "Highly efficient gene transfer into cord blood nonobese diabetic/severe combined immunodeficiency repopulating cells by oncoretroviral vector particles pseudotyped with the feline endogenous retrovirus (RD114) envelope protein", BLOOD, 96(4), 2000, pp. 1206-1214

Abstract

Limited expression of the amphotropic envelope receptor is a recognized barrier to efficient oncoretroviral vector-mediated gene transfer. Human hematopoietic cell lines and cord blood-derived CD34(+) and CD34(+), CD38(-) cell populations and the progenitors contained therein were transduced far more efficiently with oncoretroviral particles pseudotyped with the envelope protein of feline endogenous virus (RD114) than with conventional amphotropic vector particles. Similarly, human repopulating cells from umbilical cord blood capable of establishing hematopoiesis in immunodeficient mice were efficiently transduced with RD114-pseudotyped particles, whereas amphotropic particles were ineffective at introducing the proviral genome. After onlya single exposure of CD34(+) cord blood cells to RD114-pseudotyped particles, all engrafted nonobese diabetic/ severe combined immunodeficiency mice (15 of 15) contained genetically modified human bone marrow cells. Human cells that were positive for enhanced green fluorescent protein represented as much as 90% of the graft. The use of RD114-pseudotyped vectors may be advantageous for therapeutic gene transfer into hematopoietic stem cells. (Blood, 2000; 96:1206-1214) (C) 2000 by The American Society of Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 19:40:11