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Titolo:
Signaling via LAT (linker for T-cell activation) and Syk/ZAP70 is requiredfor ERK activation and NFAT transcriptional activation following CD2 stimulation
Autore:
Martelli, MP; Lin, HM; Zhang, WQ; Samelson, LE; Bierer, BE;
Indirizzi:
NHLBI, Lab Lymphocyte Biol, NIH, Bethesda, MD 20892 USA NHLBI Bethesda MDUSA 20892 Lymphocyte Biol, NIH, Bethesda, MD 20892 USA NCI, Cellular & Mol Biol Lab, NIH, Bethesda, MD 20892 USA NCI Bethesda MDUSA 20892 lar & Mol Biol Lab, NIH, Bethesda, MD 20892 USA
Titolo Testata:
BLOOD
fascicolo: 6, volume: 96, anno: 2000,
pagine: 2181 - 2190
SICI:
0006-4971(20000915)96:6<2181:SVL(FT>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
LYMPHOCYTE ANTIGEN RECEPTORS; PROTEIN TYROSINE KINASE; PHOSPHOLIPASE C-GAMMA-1; CYTOPLASMIC DOMAIN; PHOSPHORYLATION; COMPLEX; CD45; PATHWAY; JURKAT; TRANSDUCTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Bierer, BE NHLBI, Lab Lymphocyte Biol, NIH, Bldg 10,room 5D49,10 Ctr Dr, Bethesda, MD20892 USA NHLBI Bldg 10,room 5D49,10 Ctr Dr Bethesda MD USA 20892 892 USA
Citazione:
M.P. Martelli et al., "Signaling via LAT (linker for T-cell activation) and Syk/ZAP70 is requiredfor ERK activation and NFAT transcriptional activation following CD2 stimulation", BLOOD, 96(6), 2000, pp. 2181-2190

Abstract

Activation of T cells can be initiated through cell surface molecules in addition to the T-cell receptor-CD3 (TCR-CD3) complex. In human T cells, ligation of the CD2 molecule by mitogenic pairs of anti-CD2 monoclonal antibodies activates T cells via biochemical signaling pathways similar but not identical to those elicited on TCR engagement. This study describes a key role for the p36/38 membrane adapter protein linker for T cell activation (LAT) in CD2-mediated T cell activation. Following ligation of CD2 on the surface of the Jurkat T-cell line and human purified T cells, LAT was tyrosine phosphorylated and shown to associate in vivo with a number of other tyrosine phosphorylated proteins including PLC gamma-1, Grb-2, and SLP-76. Using Jurkat cell lines deficient in ZAP70/Syk (P116) or LAT (ANJ3) expression, CD2-dependent PLC gamma-1 and SLP-76 tyrosine phosphorylation required expression both of ZAP70 or Syk and of LAT. As predicted, the absence of either LAT or ZAP70/Syk kinases correlated with a defect in the induction of nuclear factor of activated T cells (NFAT) transcriptional activity, activation of the interleukin-2 promoter, and ERK phosphorylation following CD2 stimulation. These data suggest that LAT is an adapter protein important for the regulation of CD2-mediated T-cell activation. (Blood. 2000;96:2181-2190) (C)2000 by The American Society of Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 19:04:14