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Titolo:
Comprehensive gene expression profile of LPS-stimulated human monocytes bySAGE
Autore:
Suzuki, T; Hashimoto, S; Toyoda, N; Nagai, S; Yamazaki, N; Dong, HY; Sakai, J; Yamashita, T; Nukiwa, T; Matsushima, K;
Indirizzi:
Univ Tokyo, Sch Med, Dept Mol Prevent Med, Bunkyo Ku, Tokyo 1130033, JapanUniv Tokyo Tokyo Japan 1130033 vent Med, Bunkyo Ku, Tokyo 1130033, Japan Univ Tokyo, Sch Med, CREST, Bunkyo Ku, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 d, CREST, Bunkyo Ku, Tokyo 1130033, Japan Tohoku Univ, Inst Dev Aging & Canc, Dept Resp Oncol & Mol Med, Sendai, Miyagi, Japan Tohoku Univ Sendai Miyagi Japan p Oncol & Mol Med, Sendai, Miyagi, Japan
Titolo Testata:
BLOOD
fascicolo: 7, volume: 96, anno: 2000,
pagine: 2584 - 2591
SICI:
0006-4971(20001001)96:7<2584:CGEPOL>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
SERIAL ANALYSIS; BLOOD MONOCYTES; MESSENGER-RNA; SEPTIC SHOCK; LIPOPOLYSACCHARIDE; CELLS; GROWTH; PATHOGENESIS; MACROPHAGES; ADENOSINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Matsushima, K Univ Tokyo, Sch Med, Dept Mol Prevent Med, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1130033, Japan Univ Tokyo 7-3-1 Hongo Tokyo Japan 1130033 o 1130033, Japan
Citazione:
T. Suzuki et al., "Comprehensive gene expression profile of LPS-stimulated human monocytes bySAGE", BLOOD, 96(7), 2000, pp. 2584-2591

Abstract

Monocytes play a pivotal role in various human infectious and inflammatorydiseases. To reveal a whole picture of pathophysiologic function of activated human monocytes, this study used the serial analysis of gene expression(SAGE) procedure in lipopolysaccharide (LPS)-stimulated human monocytes. Atotal of 35 874 tags corresponding to more than 12 000 different transcripts were sequenced. Comparison of gene expression profile with that of resting monocytes revealed the LPS-inducible gene expression profile. Many cytokines and chemokines, including interleukin (IL)-6, IL-1 alpha, IL-1 beta, tumor necrosis factor (TNF)-alpha, macrophage inflammatory protein (MIP)-1 beta, MIP-2 beta, MIP-2 alpha, liver and activation-regulated chemokine (LARC), MIP-1 alpha, thymus and activation-regulated chemokine (TARC), macrophage-derived chemokine (MDC), regulated on activation, normal T cell expressed and secreted (RANTES), growth-regulated oncogene (GRO) alpha, and IL-8, were observed in the highest inducible transcripts. Other genes encoding plasminogen activator inhibitor type 2 (PAI-2), Hc-gp39, apolipoproteins, malate dehydrogenase, matrix metalloproteinase-9 (MMP-9), and cyclooxygenase (COX2) were also highly elevated in LPS-stimulated monocytes. Moreover, upregulation of Naf1 beta, IL-7 receptor, adenosine receptor A2a, and many novelgenes was newly identified. These results suggest that the LPS-inducible gene products may be involved In cell activation and migration, angiogenesis, tissue remodeling, and metabolism, and thus may orchestrate the inflammatory reactions. On the other hand, the expression of numerous sets of novel genes was discovered to be down-regulated on LPS stimulation. This study represents the first comprehensive analysis of LPS-inducible gene expression in human monocytes and provides tremendous novel information for the function of LPS-activated monocytes and targets for diagnosing, monitoring, and treating sepsis and various human infectious and inflammatory diseases.(Blood. 2000;96:2584-2591) (C) 2000 by The American Society of Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 12:44:17