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Titolo:
Osmoprotective effect of glycine betaine on foreign protein production in hyperosmotic recombinant Chinese hamster ovary cell cultures differs among cell lines
Autore:
Ryu, JS; Kim, TK; Chung, JY; Lee, GM;
Indirizzi:
Korea Adv Inst Sci & Technol, Dept Biol Sci, Yusong Gu, Taejon 305701, South Korea Korea Adv Inst Sci & Technol Taejon South Korea 305701 5701, South Korea
Titolo Testata:
BIOTECHNOLOGY AND BIOENGINEERING
fascicolo: 2, volume: 70, anno: 2000,
pagine: 167 - 175
SICI:
0006-3592(20001020)70:2<167:OEOGBO>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOCLONAL-ANTIBODY PRODUCTION; HYBRIDOMA CELLS; OSMOTIC-PRESSURE; PRODUCTION KINETICS; SODIUM-BUTYRATE; STRESS; GROWTH; EXPRESSION; EVOLUTION; OSMOLYTES;
Keywords:
foreign protein production; glycine betaine; hyperosmotic pressure; recombinant Chinese hamster ovary (rCHO) cells; specific productivity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Lee, GM Korea Adv Inst Sci & Technol, Dept Biol Sci, Yusong Gu, 373-1 Kusong Dong,Taejon 305701, South Korea Korea Adv Inst Sci & Technol 373-1 Kusong Dong Taejon South Korea 305701
Citazione:
J.S. Ryu et al., "Osmoprotective effect of glycine betaine on foreign protein production in hyperosmotic recombinant Chinese hamster ovary cell cultures differs among cell lines", BIOTECH BIO, 70(2), 2000, pp. 167-175

Abstract

When three recombinant Chinese hamster ovary (rCHO) cell lines, CHO/dhfr-B22-4, CS13-1.00*, and CS13-0.02*, were cultivated in hyperosmolar media resulting from NaCl addition, their specific foreign protein productivity increased with medium osmolality. However, due to a simultaneous suppression ofcell growth at elevated osmolality, no enhancement in the maximum foreign protein titer was made in batch cultures. To test the feasibility of using glycine betaine, known as a strong osmoprotective compound, for improved foreign protein production in hyperosmotic rCHO cell cultures, hyperosmotic batch cultures were carried out in the presence of 15 mM glycine betaine. Glycine betaine was found to have a strong osmoprotective effect on all threerCHO cell lines. Inclusion of 15 mM glycine betaine in hyperosmolar mediumenabled rCHO cell lines to grow at 557 to 573 mOsm/kg, whereas they could not grow in the absence of glycine betaine. However, effect of glycine betaine inclusion in hyperosmolar medium on foreign protein production differedamong rCHO cell lines. CHO/dhfr-B22-4 cells retained enhanced specific human thrombopoietin (hTPO) productivity in the presence of glycine betaine, and thereby the maximum hTPO titer obtained at 573 mOsm/kg was increased by 72% over that obtained in the control culture with physiological osmolality(292 mOsm/kg). On the other hand, enhanced specific antibody productivity of CS13-1.00* and CS13-0.02* at elevated osmolality was decreased significantly in the presence of glycine betaine. As a result, the maximum antibody titer at 557 mOsm/kg was similar to that obtained in the control culture with physiological osmolality. The mRNA contents per cell determined by northern blot hybridization correlated with q in all three rCHO cell lines, indicating that transcriptional regulation is responsible in part for q enhancement at hyperosmolality in the absence as well as the presence of glycine betaine. Taken together, efficacy of the simultaneous use of hyperosmotic pressure and glycine betaine as a means to improve foreign protein productionwas variable among different rCHO cell lines. (C) 2000 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/01/20 alle ore 16:33:19