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Titolo:
Evidence for a Menkes-like protein with a nuclear targeting sequence
Autore:
Reddy, MCM; Majumdar, S; Harris, ED;
Indirizzi:
Texas A&M Univ, Dept Biochem & Biophys, College Stn, TX 77843 USA Texas A&M Univ College Stn TX USA 77843 iophys, College Stn, TX 77843 USA Texas A&M Univ, Fac Nutr, College Stn, TX 77843 USA Texas A&M Univ College Stn TX USA 77843 c Nutr, College Stn, TX 77843 USA
Titolo Testata:
BIOCHEMICAL JOURNAL
, volume: 350, anno: 2000,
parte:, 3
pagine: 855 - 863
SICI:
0264-6021(20000915)350:<855:EFAMPW>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
CANDIDATE GENE; GOLGI LOCALIZATION; MESSENGER-RNA; DISEASE; TSG101; TRANSCRIPTS; TRANSPORT; ENCODES; ATPASE; ATP7A;
Keywords:
alternative splicing; ATP7A mRNA; copper transport; Menkes disease gene; nuclear-localization sequence;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Harris, ED Texas A&M Univ, Dept Biochem & Biophys, 2128 TAMUS, College Stn, TX 77843 USA Texas A&M Univ 2128 TAMUS College Stn TX USA 77843 TX 77843 USA
Citazione:
M.C.M. Reddy et al., "Evidence for a Menkes-like protein with a nuclear targeting sequence", BIOCHEM J, 350, 2000, pp. 855-863

Abstract

Extracts from three human cell lines were found to contain abridged Menkesdisease gene transcripts with novel insertion sequences. The transcript variant that is the focus of the present study codes for a 103-residue protein containing the first heavy-metal-binding domain (Hmbl) of ATP7A, the Cu-ATPase associated with Menkes disease. This transcript variant has a 45-bp nucleotide insert interposed between exons 1 and 2 of ATP7A that starts witha 5' ATG that is in-frame with the downstream ATG translation start site of ATP7A. We report here that the 66-bp nucleotides positioned between the upstream and downstream ATG sites encode 22 amino acid residues whose primary structure in part meets the criteria for a nuclear-localization sequence (NLS). We have referred to the transcript as nuclear Menkes-like (NML) 45. A green fluorescent protein (GFP) construct with NML45 when transfected in Chinese hamster ovary cells localized to the cell nucleus. A similar construct without the 66-bp segment exhibited a random dispersed fluorescent pattern in the cytosol. GFP constructs encoding ATP7A exons likewise failed to direct GFP into the cell nucleus, suggesting the nuclear determinant is notin an internal domain of the protein. The data suggest that the 22-residuesegment contains an NLS for an 11.2-kDa protein with one Cu-binding site that may function as a chaperone to transport Cu into the nucleus of mammalian cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 15:33:21