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Titolo:
The PCNA from Thermococcus fumicolans functionally interacts with DNA polymerase delta
Autore:
Henneke, G; Raffin, JP; Ferrari, E; Jonsson, ZO; Dietrich, J; Hubscher, U;
Indirizzi:
IFREMER, Lab Biotechnol Microorganismes Hydrothermaux, DRV, VP, F-29280 Plouzane, France IFREMER Plouzane France F-29280 rmaux, DRV, VP, F-29280 Plouzane, France Univ Zurich, Inst Vet Biochem, CH-8057 Zurich, Switzerland Univ Zurich Zurich Switzerland CH-8057 chem, CH-8057 Zurich, Switzerland
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 2, volume: 276, anno: 2000,
pagine: 600 - 606
SICI:
0006-291X(20000924)276:2<600:TPFTFF>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL NUCLEAR ANTIGEN; COMPLETE GENOME SEQUENCE; HYPERTHERMOPHILIC ARCHAEON; SULFOLOBUS-SOLFATARICUS; SLIDING CLAMPS; REPLICATION; BINDING; EPSILON; REPAIR; ALPHA;
Keywords:
PCNA; hyperthermophile; archaea; Thermococcus fumicolans; conservation; DNA polymerase delta;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Henneke, G IFREMER, Lab Biotechnol Microorganismes Hydrothermaux, DRV, VP,BP 70, F-29280 Plouzane, France IFREMER BP 70 Plouzane France F-29280 F-29280 Plouzane, France
Citazione:
G. Henneke et al., "The PCNA from Thermococcus fumicolans functionally interacts with DNA polymerase delta", BIOC BIOP R, 276(2), 2000, pp. 600-606

Abstract

We have cloned the gene encoding proliferating cell nuclear antigen (PCNA)from the hyperthermophilic euryarchaeote Thermococcus fumicolans (Tfu). Tfu PCNA contains 250 amino acids with a calculated M-r of 28,000 and is 26% identical to human PCNA. Next, Tfu PCNA was overexpressed in Escherichia coli and it showed an apparent molecular mass of 33.5 kDa. The purified Tfu PCNA was tested first with recombinant Tfu DNA polymerase I (Tfu pol) and second with calf thymus DNA polymerase delta (pol delta). When tested with the homologous Tfu pol on bacteriophage lambda DNA, large amounts of Tfu PCNAwere required to obtain two- to threefold stimulation. Surprisingly, however, Tfu PCNA was much more efficient than human PCNA in stimulating calf thymus pol delta. Our data suggest that PCNA has been functionally conserved not only within eukaryotes but also from hyperthermophilic euryarchaeotes to mammals. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 18:22:38