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Titolo:
Effects of copper on cortisol receptor and metallothionein expression in gills of Oncorhynchus mykiss
Autore:
Dang, ZC; Flik, G; Ducouret, B; Hogstrand, C; Bonga, SEW; Lock, RAC;
Indirizzi:
Catholic Univ Nijmegen, Dept Anim Physiol, NL-6525 ED Nijmegen, Netherlands Catholic Univ Nijmegen Nijmegen Netherlands NL-6525 ED egen, Netherlands Univ Rennes 1, F-35042 Rennes, France Univ Rennes 1 Rennes France F-35042 niv Rennes 1, F-35042 Rennes, France Univ London Kings Coll, Sch Life & Hlth Sci, Div Life Sci, London SE1 8WA,England Univ London Kings Coll London England SE1 8WA ci, London SE1 8WA,England
Titolo Testata:
AQUATIC TOXICOLOGY
fascicolo: 1, volume: 51, anno: 2000,
pagine: 45 - 54
SICI:
0166-445X(200011)51:1<45:EOCOCR>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
TILAPIA OREOCHROMIS-MOSSAMBICUS; WATER RAINBOW-TROUT; GLUCOCORTICOID-RECEPTOR; FRESH-WATER; DOWN-REGULATION; SALMO-GAIRDNERI; GENE-EXPRESSION; CHLORIDE CELLS; COHO SALMON; HALF-LIFE;
Keywords:
glucocorticoid receptor; metallothionein; fish gills; chloride cell; Cu; immunocytochemistry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Lock, RAC Catholic Univ Nijmegen, Dept Anim Physiol, Toernoolveld, NL-6525ED Nijmegen, Netherlands Catholic Univ Nijmegen Toernoolveld Nijmegen Netherlands NL-6525 ED
Citazione:
Z.C. Dang et al., "Effects of copper on cortisol receptor and metallothionein expression in gills of Oncorhynchus mykiss", AQUAT TOX, 51(1), 2000, pp. 45-54

Abstract

Effects of waterborne Cu (2.4 mu M) on the expression of glucocorticoid receptor (GR) and metallothionein (MT) in the branchial epithelium of freshwater rainbow trout (Oncorhynchus mykiss) was studied by immunocytochemistry. After 5 days of Cu exposure, the number of GR-immunoreactive (GR-ir) cellsin the gill epithelium had decreased, whereas the number of MT-ir cells had increased. Localization of GR in chloride cells was achieved by double staining for Na+/K+-ATPase; other cell types were identified on the basis of their topology. GRs were present in the chloride cells in both the filaments and lamellae, in respiratory cells in the lamellae, in pavement cells, basal layer cells and undifferentiated cells in the filaments. Co-localization of Na+/K+-ATPase and MT revealed that MT was expressed in chloride cells,both in filaments and lamellae. Occasionally, MT immunoreactivity was found in pavement cells and in undifferentiated cells. By double staining for Na+/K+-ATPase and GR, for Na+/K+-ATPase and MT and for GR and MT, we can conclude that after 5 days of Cu stress there are chloride cells that express CR and MT, GR or MT alone or neither of the two proteins. This apparent functional heterogeneity of branchial chloride cells may reflect a limited window when chloride cell subpopulations show an adaptive response to Cu. (C) 2000 Elsevier Science B.V. All rights reserved.

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Documento generato il 25/11/20 alle ore 04:47:17