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Titolo:
Cell transplantation causes loss of gap junctions and activates GGT expression permanently in host liver
Autore:
Gupta, S; Rajvanshi, P; Malhi, H; Slehria, S; Sokhi, RP; Vasa, SRG; Dabeva, M; Shafritz, DA; Kerr, A;
Indirizzi:
Albert Einstein Coll Med, Marion Bessin Liver Res Ctr, Bronx, NY 10461 USAAlbert Einstein Coll Med Bronx NY USA 10461 Res Ctr, Bronx, NY 10461 USA Albert Einstein Coll Med, Comprehens Canc Res Ctr, Bronx, NY 10461 USA Albert Einstein Coll Med Bronx NY USA 10461 Res Ctr, Bronx, NY 10461 USA Albert Einstein Coll Med, Dept Med, Bronx, NY 10461 USA Albert Einstein Coll Med Bronx NY USA 10461 Dept Med, Bronx, NY 10461 USA Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY 10461 USA Albert Einstein Coll Med Bronx NY USA 10461 ell Biol, Bronx, NY 10461 USA Albert Einstein Coll Med, Dept Pathol, Bronx, NY 10461 USA Albert EinsteinColl Med Bronx NY USA 10461 t Pathol, Bronx, NY 10461 USA Albert Einstein Coll Med, Dept Radiol, Bronx, NY 10461 USA Albert EinsteinColl Med Bronx NY USA 10461 t Radiol, Bronx, NY 10461 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
fascicolo: 4, volume: 279, anno: 2000,
pagine: G815 - G826
SICI:
0193-1857(200010)279:4<G815:CTCLOG>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAMMA-GLUTAMYL-TRANSPEPTIDASE; CARCINOGEN-TREATED RATS; EPIDERMAL GROWTH-FACTOR; EPITHELIAL-CELLS; GENE-THERAPY; HEPATOCYTE TRANSPLANTATION; CONNEXIN-32 EXPRESSION; TRANSGENE EXPRESSION; PROGENITOR CELLS; OVAL CELLS;
Keywords:
hepatocyte; ischemia; injury; gene expression; vasodilatation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Gupta, S Albert Einstein Coll Med, Marion Bessin Liver Res Ctr, Ullmann 625, Bronx,NY 10461 USA Albert Einstein Coll Med Ullmann 625 Bronx NY USA 10461 10461 USA
Citazione:
S. Gupta et al., "Cell transplantation causes loss of gap junctions and activates GGT expression permanently in host liver", AM J P-GAST, 279(4), 2000, pp. G815-G826

Abstract

Cell transplantation into hepatic sinusoids, which is necessary for liver repopulation, could cause hepatic ischemia. To examine the effects of cell transplantation on host hepatocytes, we transplanted Fisher 344 rat hepatocytes into syngeneic dipeptidyl peptidase IV-deficient rats. Within 24 h of cell transplantation, areas of ischemic necrosis, along with transient disruption of gap junctions, appeared in the liver. Moreover, host hepatocytes expressed gamma-glutamyl transpeptidase (GGT) extensively, which was observed even 2 years after cell transplantation. GGT expression was not associated with alpha-fetoprotein activation, which is present in progenitor cells. Increased GGT expression was apparent after transplantation of nonparenchymal cells and latex beads but not after injection of saline, fragmented hepatocytes, hepatocyte growth factor, or turpentine. Some host hepatocytes exhibited apoptosis, as well as DNA synthesis, between 24 and 48 h after celltransplantation. Changes in gap junctions, GGT expression, DNA synthesis, and apoptosis after cell transplantation were prevented by vasodilators. The findings indicated the onset of ischemic liver injury after cell transplantation. These hepatic perturbations must be considered when transplanted cells are utilized as reporters for biological studies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/10/20 alle ore 11:34:14