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Titolo:
Expression of P2X(7) purinoceptors on human lymphocytes and monocytes: evidence for nonfunctional P2X(7) receptors
Autore:
Gu, BJ; Zhang, WY; Bendall, LJ; Chessell, IP; Buell, GN; Wiley, JS;
Indirizzi:
Univ Sydney, Nepean Hosp, Dept Med, Penrith, NSW 2750, Australia Univ Sydney Penrith NSW Australia 2750 Med, Penrith, NSW 2750, Australia Univ Cambridge, Dept Pharmacol, Cambridge CB2 1QJ, England Univ CambridgeCambridge England CB2 1QJ col, Cambridge CB2 1QJ, England Geneva Inst Biomed Res, Dept Mol Biol, CH-1228 Geneva, Switzerland Geneva Inst Biomed Res Geneva Switzerland CH-1228 28 Geneva, Switzerland
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
fascicolo: 4, volume: 279, anno: 2000,
pagine: C1189 - C1197
SICI:
0363-6143(200010)279:4<C1189:EOPPOH>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
GATED ION CHANNELS; EXTRACELLULAR ATP; L-SELECTIN; PLASMA-MEMBRANE; PURINERGIC RECEPTORS; P2Z PURINORECEPTOR; PHOSPHOLIPASE-D; LEUKEMIA CELLS; ACTIVATION; MACROPHAGES;
Keywords:
extracellular adenosine 5 '-triphosphate; adenosine 5 '-triphosphate-induced ethidium uptake; monocyte P2X(7); lymphocyte P2X(7); B cell chronic lymphocytic leukemia;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Wiley, JS Univ Sydney, Nepean Hosp, Dept Med, Clin Sci Bldg, Penrith, NSW 2750, Australia Univ Sydney Clin Sci Bldg Penrith NSW Australia 2750 , Australia
Citazione:
B.J. Gu et al., "Expression of P2X(7) purinoceptors on human lymphocytes and monocytes: evidence for nonfunctional P2X(7) receptors", AM J P-CELL, 279(4), 2000, pp. C1189-C1197

Abstract

Lymphocytes from normal subjects and patients with B-chronic lymphocytic leukemia (B-CLL) show functional responses to extracellular ATP characteristic of the P2X(7) receptor (previously termed P2Z). These responses include opening of a cation-selective channel/pore that allows entry of the fluorescent dye ethidium and activation of a membrane metalloprotease that sheds the adhesion molecule L-selectin. The surface expression of P2X(7) receptorswas measured in normal leucocytes, platelets, and B-CLL lymphocytes and correlated with their functional responses. Monocytes showed four- to fivefold greater expression of P2X(7) than B, T, and NK lymphocytes, whereas P2X(7) expression on neutrophils and platelets was weak. All cell types demonstrated abundant intracellular expression of this receptor. All 12 subjects with B-CLL expressed lymphocyte P2X(7) at about the same level as B lymphocytes from normal subjects. P2X(7) function, measured by ATP-induced uptake ofethidium, correlated closely with surface expression of this receptor in normal and B-CLL lymphocytes and monocytes (n = 47, r = 0.70; P< 0.0001). However, in three patients the ATP-induced uptake of ethidium into the malignant B lymphocytes was low or absent. The lack of P2X(7) function in these Blymphocytes was confirmed by the failure of ATP to induce Ba2+ uptake intotheir lymphocytes. This lack of function of the P2X(7) receptor resulted in a failure of ATP-induced shedding of L-selectin, an adhesion molecule that directs the recirculation of lymphocytes from blood into the lymph node.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 09:20:54