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Titolo:
Desmin knockout muscles generate lower stress and are less vulnerable to injury compared with wild-type muscles
Autore:
Sam, M; Shah, S; Friden, J; Milner, DJ; Capetanaki, Y; Lieber, RL;
Indirizzi:
Univ Calif San Diego, Dept Orthopaed, San Diego, CA 92161 USA Univ Calif San Diego San Diego CA USA 92161 paed, San Diego, CA 92161 USA Univ Calif San Diego, Dept Bioengn, San Diego, CA 92161 USA Univ Calif SanDiego San Diego CA USA 92161 engn, San Diego, CA 92161 USA Univ Calif San Diego, Dept Appl Mech & Engn Sci, Biomed Sci Grad Grp, San Diego, CA 92161 USA Univ Calif San Diego San Diego CA USA 92161 Grp, San Diego, CA 92161 USA Vet Adm Med Ctr, San Diego, CA 92161 USA Vet Adm Med Ctr San Diego CA USA92161 m Med Ctr, San Diego, CA 92161 USA Sahlgrens Univ Hosp, Dept Hand Surg, S-41345 Gothenburg, Sweden Sahlgrens Univ Hosp Gothenburg Sweden S-41345 S-41345 Gothenburg, Sweden Baylor Coll Med, Dept Cell Biol, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 ept Cell Biol, Houston, TX 77030 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
fascicolo: 4, volume: 279, anno: 2000,
pagine: C1116 - C1122
SICI:
0363-6143(200010)279:4<C1116:DKMGLS>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
MICE LACKING DESMIN; SKELETAL-MUSCLE; ECCENTRIC CONTRACTIONS; ARCHITECTURE; DISRUPTION; EXERCISE;
Keywords:
intermediate filaments; cytoskeletal; muscle injury; biomechanics; aging;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Lieber, RL Univ Calif San Diego, Sch Med, Dept Orthopaed 9151, San Diego, CA 92161 USA Univ Calif San Diego San Diego CA USA 92161 iego, CA 92161 USA
Citazione:
M. Sam et al., "Desmin knockout muscles generate lower stress and are less vulnerable to injury compared with wild-type muscles", AM J P-CELL, 279(4), 2000, pp. C1116-C1122

Abstract

The functional role of the skeletal muscle intermediate filament system was investigated by measuring the magnitude of muscle force loss after cycliceccentric contraction (EC) in normal and desmin null mouse extensor digitorum longus muscles. Isometric stress generated was significantly greater inwildtype (313 +/- 8 kPa) compared with knockout muscles (276 +/- 13 kPa) before EC (P< 0.05), but 1 h after 10 ECs, both muscle types generated identical levels of stress (similar to 250 kPa), suggesting less injury to the knockout. Differences in injury susceptibility were not explained by the different absolute stress levels imposed on wild-type versus knockout muscles (determined by testing older muscles) or by differences in fiber length or mechanical energy absorbed. Morphometric analysis of longitudinal electron micrographs indicated that Z disks from knockout muscles were more staggered (0.36 +/- 0.03 mu m) compared with wild-type muscles (0.22 +/- 0.03 mu m), which may indicate that the knockout cytoskeleton is more compliant. These data demonstrate that lack of the intermediate filament system decreases isometric stress production and that the desmin knockout muscle is less vulnerable to mechanical injury.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 20:26:53