Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Oxidants and regulation of K+-Cl- cotransport in equine red blood cells
Autore:
Muzyamba, MC; Speake, PF; Gibson, JS;
Indirizzi:
Univ Liverpool, Dept Vet Preclin Sci, Liverpool L69 7ZJ, Merseyside, England Univ Liverpool Liverpool Merseyside England L69 7ZJ , Merseyside, England
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
fascicolo: 4, volume: 279, anno: 2000,
pagine: C981 - C989
SICI:
0363-6143(200010)279:4<C981:OAROKC>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDUCED HEMOLYTIC-ANEMIA; HEINZ BODY FORMATION; SICKLE ERYTHROCYTES; OXYGEN-AFFINITY; MEMBRANE; HEMOGLOBIN; TRANSPORT; SHEEP; CHLORIDE; NITRITE;
Keywords:
oxygen; nitrite; 1-chloro-2,4-dinitrobenzene; erythrocytes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Gibson, JS Univ London St Georges Hosp, Sch Med, Dept Physiol, Cranmer Terrace, London SW17 0RE, England Univ London St Georges Hosp Cranmer Terrace London England SW17 0RE
Citazione:
M.C. Muzyamba et al., "Oxidants and regulation of K+-Cl- cotransport in equine red blood cells", AM J P-CELL, 279(4), 2000, pp. C981-C989

Abstract

The effect of oxidants on K+-Cl- cotransport (KCC) was investigated in equine red blood cells. Carbon monoxide mimicked O-2. The substituted benzaldehyde, 12C79 (5 mM), markedly increased O-2 affinity. In N-2, however, O-2 saturation was low (<10%) but KCC remained active. Nitrite (NO2-) oxidized heme to methemoglobin (metHb). High concentrations of NO2- (1 and 5 mM vs. 0.5 mM) increased KCC activity above control levels; it became O-2 independent but remained sensitive to other stimuli. 1-Chloro-2,4-dinitrobenzene (1-3 mM) depleted reduced glutathione (GSH). Prolonged exposure (60-120 min, 1mM) or high concentrations (3 mM) stimulated an O-2-independent KCC activity; short exposures and low concentrations (30 min, 0.5 or 1 mM) did not. The effect of these manipulations was correlated with changes in GSH and metHb concentrations. An oxy conformation of Hb was necessary for KCC activation. An increase in its activity over the level found in oxygenated control cells required both accumulation of metHb and depletion of GSH. Findings are relevant to understanding the physiology and pathology of regulation of KCC.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 09:02:44