Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Evaluation of the CaMAL2 promoter for regulated expression of genes in Candida albicans
Autore:
Backen, AC; Broadbent, ID; Fetherston, RW; Rosamond, JDC; Schnell, NF; Stark, MJR;
Indirizzi:
Univ Dundee, Dept Biochem, Dundee DD1 5EH, Scotland Univ Dundee Dundee Scotland DD1 5EH pt Biochem, Dundee DD1 5EH, Scotland Univ Manchester, Manchester M13 9PT, Lancs, England Univ Manchester Manchester Lancs England M13 9PT M13 9PT, Lancs, England AstraZeneca Pharmaceut, Macclesfield SK10 4TG, Cheshire, England AstraZeneca Pharmaceut Macclesfield Cheshire England SK10 4TG re, England
Titolo Testata:
YEAST
fascicolo: 12, volume: 16, anno: 2000,
pagine: 1121 - 1129
SICI:
0749-503X(20000915)16:12<1121:EOTCPF>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
OROTIDINE-5'-PHOSPHATE DECARBOXYLASE; SACCHAROMYCES-CEREVISIAE; REPLICATION; SEQUENCE;
Keywords:
CIp10; MAL2; Candida; expression vector; RP10;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Stark, MJR Univ Dundee, Dept Biochem, MSI WTB Complex, Dundee DD1 5EH, Scotland Univ Dundee MSI WTB Complex Dundee Scotland DD1 5EH , Scotland
Citazione:
A.C. Backen et al., "Evaluation of the CaMAL2 promoter for regulated expression of genes in Candida albicans", YEAST, 16(12), 2000, pp. 1121-1129

Abstract

An expression vector (CIp10-MAL2p) for use in Candida albicans has been constructed in which a gene of interest can be placed under the control of the CaMAL2 maltase promoter and stably integrated at the CaRP10 locus. Using this vector to express the Candida URA3 gene from the CaMAL2 promoter, we have demonstrated tight regulation of CaUXA3 expression by carbon source. Thus under conditions when the CaMAL2 promoter is not induced, expression of Candida URA3 was unable either to complement a C. albicans ura3 mutation orto confer sensitivity to 5-fluoroorotic acid, a compound which is highly toxic to URA3 strains. Since Candida albicans is an obligate diploid organism, analysis of gene function requires manipulation of both copies of any gene of interest. Our expression vector provides a strategy by which the remaining copy of a gene of interest can be placed under CaMAL2 promoter control in a strain where the first copy has been deleted, permitting analysis ofgene function by manipulation of carbon source. CIp10-MAL2p should therefore provide a useful means for functional analysis of genes in C, albicans, We have used this strategy with C, albicans DPB2 to demonstrate that the gene is essential and that loss of function leads cells to adopt a hypha-likemorphology as they cease proliferation. Copyright (C) 2000 John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 23:34:57