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Titolo:
Systemic induction of a Phytolacca insularis antiviral protein gene by mechanical wounding, jasmonic acid, and abscisic acid
Autore:
Song, SK; Choi, Y; Moon, YH; Kim, SG; Choi, YD; Lee, JS;
Indirizzi:
Seoul Natl Univ, Dept Mol Biol, Seoul 151742, South Korea Seoul Natl UnivSeoul South Korea 151742 Biol, Seoul 151742, South Korea Seoul Natl Univ, Res Ctr Cell Differentiat, Seoul 151742, South Korea Seoul Natl Univ Seoul South Korea 151742 tiat, Seoul 151742, South Korea Seoul Natl Univ, Dept Biol, Seoul 151742, South Korea Seoul Natl Univ Seoul South Korea 151742 Biol, Seoul 151742, South Korea Seoul Natl Univ, Dept Agr Chem, Suwon 441744, South Korea Seoul Natl UnivSuwon South Korea 441744 Chem, Suwon 441744, South Korea
Titolo Testata:
PLANT MOLECULAR BIOLOGY
fascicolo: 4, volume: 43, anno: 2000,
pagine: 439 - 450
SICI:
0167-4412(200007)43:4<439:SIOAPI>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
RIBOSOME-INACTIVATING PROTEINS; RICIN A-CHAIN; DISEASE RESISTANCE; METHYL JASMONATE; VIRUS-RESISTANCE; PLANTS; EXPRESSION; RNA; DEFENSE; ACTIVATION;
Keywords:
jasmonic acid; Phytolacca insularis antiviral protein (PIP); ribosome-inactivating protein (RIP); wound-inducible expression;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Lee, JS Seoul Natl Univ, Dept Mol Biol, Seoul 151742, South Korea Seoul Natl Univ Seoul South Korea 151742 oul 151742, South Korea
Citazione:
S.K. Song et al., "Systemic induction of a Phytolacca insularis antiviral protein gene by mechanical wounding, jasmonic acid, and abscisic acid", PLANT MOL B, 43(4), 2000, pp. 439-450

Abstract

We have isolated a gene encoding a ribosome-inactivating protein (RIP) from Phytolacca insularis, designated as P. insularis antiviral protein 2 (PIP2). The PIP2 gene contained an open reading frame encoding a polypeptide of315 amino acids. The deduced amino acid sequence of PIP2 was similar to those of other RIPs from Phytolacca plants. Recombinant PIP2 was expressed inEscherichia coli and was used to investigate its biological activities. Recombinant PIP2 inhibited protein synthesis in rabbit reticulocyte lysate byinactivating ribosomes through N-glycosidase activity. It also exhibited antiviral activity against tobacco mosaic virus (TMV). Expression of the PIP2 gene was developmentally regulated in leaves and roots of P. insularis. Furthermore, expression of the PIP2 gene was induced in leaves by mechanicalwounding. The wound induction of the PIP2 gene was systemic. Expression ofthe PIP2 gene also increased in leaves in a systemic manner after treatment with jasmonic acid (JA) and abscisic acid (ABA), but not with salicylic acid (SA). These results imply that plants have employed the systemic synthesis of the defensive proteins to protect themselves more efficiently from infecting viruses.

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Documento generato il 24/11/20 alle ore 14:29:10