Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Interaction of ResD with regulatory regions of anaerobically induced genesin Bacillus subtilis
Autore:
Nakano, MM; Zhu, Y; LaCelle, M; Zhang, XH; Hulett, FM;
Indirizzi:
Oregon Grad Inst Sci & Technol, Dept Biochem & Mol Biol, Beaverton, OR 97006 USA Oregon Grad Inst Sci & Technol Beaverton OR USA 97006 erton, OR 97006 USA Louisiana State Univ, Med Ctr, Dept Biochem & Mol Biol, Shreveport, LA 71130 USA Louisiana State Univ Shreveport LA USA 71130 ol, Shreveport, LA 71130 USA Univ Illinois, Dept Biol Sci, Mol Biol Lab, Chicago, IL 60607 USA Univ Illinois Chicago IL USA 60607 i, Mol Biol Lab, Chicago, IL 60607 USA
Titolo Testata:
MOLECULAR MICROBIOLOGY
fascicolo: 5, volume: 37, anno: 2000,
pagine: 1198 - 1207
SICI:
0950-382X(200009)37:5<1198:IORWRR>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
SIGNAL-TRANSDUCTION NETWORK; NITRITE REDUCTASE; OXYGEN LIMITATION; IDENTIFICATION; GROWTH; PHOSPHORYLATION; ACTIVATION; OMPR; FNR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Nakano, MM Oregon Grad Inst Sci & Technol, Dept Biochem & Mol Biol, 20000 NW Walker Rd, Beaverton, OR 97006 USA Oregon Grad Inst Sci & Technol 20000 NW Walker Rd Beaverton OR USA 97006
Citazione:
M.M. Nakano et al., "Interaction of ResD with regulatory regions of anaerobically induced genesin Bacillus subtilis", MOL MICROB, 37(5), 2000, pp. 1198-1207

Abstract

The two-component regulatory proteins ResD and ResE are required for anaerobic nitrate respiration in Bacillus subtilis. ResD, when it undergoes ResE-dependent phosphorylation, is thought to activate transcriptionally anaerobically induced genes such as fnr, hmp and nasD. In this report, deletion analysis of the fnr, hmp and nasD promoter regions was carried out to identify cis-acting sequences required for ResDE-dependent transcription. The results suggest that the hmp and nasD promoters have multiple target sequencesfor ResDE-dependent regulation and that fnr has a single target site. Gel mobility shift assays and DNase I footprinting analyses were performed to determine whether ResD interacts directly with the regulatory regions of thethree genes. Our results indicate that ResD specifically binds to sequences residing upstream of the hmp and nasD promoters and that phosphorylation of ResD significantly stimulates this binding. In contrast, a higher concentration of ResD is required for binding to the fnr promoter region and no stimulation of the binding by ResD phosphorylation was observed. Taken together, these results suggest that ResD activates transcription of fnr, hmp and nasD by interacting with DNA upstream of these promoters. Our results suggest that phosphorylation of ResD stimulates binding to multiple ResD binding sites, but is much less stimulatory if only a single binding site exists.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/11/20 alle ore 19:47:03