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Titolo:
Auto-transporter A protein of Neisseria meningitidis: a patent CD4(+) T-cell and B-cell stimulating antigen detected by expression cloning
Autore:
Ait-Tahar, K; Wooldridge, KG; Turner, DPJ; Atta, M; Todd, I; AlaAldeen, DAA;
Indirizzi:
Univ Nottingham, Div Microbiol, Meningococcal Res Grp, Nottingham NG7 2UH,England Univ Nottingham Nottingham England NG7 2UH p, Nottingham NG7 2UH,England Univ Nottingham, Div Immunol, Meningococcal Res Grp, Nottingham NG7 2UH, England Univ Nottingham Nottingham England NG7 2UH , Nottingham NG7 2UH, England
Titolo Testata:
MOLECULAR MICROBIOLOGY
fascicolo: 5, volume: 37, anno: 2000,
pagine: 1094 - 1105
SICI:
0950-382X(200009)37:5<1094:AAPONM>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEMBRANE VESICLE VACCINE; HUMAN TRANSFERRIN; ESCHERICHIA-COLI; IDENTIFICATION; RESPONSES; SEQUENCE; DESIGN; MEMORY; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Ala'Aldeen, DAA Univ Nottingham, Div Microbiol, Meningococcal Res Grp, Nottingham NG7 2UH,England Univ Nottingham Nottingham England NG7 2UH 7 2UH,England
Citazione:
K. Ait-Tahar et al., "Auto-transporter A protein of Neisseria meningitidis: a patent CD4(+) T-cell and B-cell stimulating antigen detected by expression cloning", MOL MICROB, 37(5), 2000, pp. 1094-1105

Abstract

A meningococcal genomic expression library was screened for potent CD4+ T-cell antigens, using patients' peripheral blood lymphocytes (PBLs). One of the most promising positive clones was fully characterized, The recombinantmeningococcal DNA contained a single, incomplete, open reading frame (ORF), which was fully reconstructed with reference to available genomic sequence data. The gene was designated autA (auto-transporter A) as its peptide sequence shares molecular characteristics of the auto-transporter family of proteins. Only a single copy of this gene was detected in the meningococcal,and none in the gonococcal, genomic sequence databases. The complete autA gene, when cloned into an expression vector, expressed a protein of approximately 68 kDa, Purified rAutA recalled strong secondary T-cell responses inPBLs of patients and some healthy donors, and induced strong primary T-cell responses in healthy donors. The human B-cell immunogenicity and cross-reactivity of AutA, purified under native conditions, was confirmed in dot immunoblot experiments. Immunoblots with rabbit polyclonal antibodies to rAutA demonstrated the conserved nature, antigenicity and cross-reactivity of AutA amongst meningococci of different serogroups and strains representing different hypervirulent lineages. AutA showed homology with another meningococcal and gonococcal ORF (designated AutB), AutB was cloned and expressed and used to raise an autB-specific antiserum. Immunoblot experiments indicated that AutB is not expressed in meningococci and does not cross-react withAutA, Thus, AutA, being a potent CD4(+) T-cell and B-cell-stimulating antigen, which is highly conserved, deserves further investigation as a potential vaccine candidate.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 14:56:10