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Titolo:
MATRIX-ASSISTED LASER DESORPTION IONIZATION MASS-SPECTROMETRY SAMPLE PREPARATION TECHNIQUES DESIGNED FOR VARIOUS PEPTIDE AND PROTEIN ANALYTES/
Autore:
KUSSMANN M; NORDHOFF E; RAHBEKNIELSEN H; HAEBEL S; ROSSELLARSEN M; JAKOBSEN L; GOBOM J; MIRGORODSKAYA E; KROLLKRISTENSEN A; PALM L; ROEPSTORFF P;
Indirizzi:
ODENSE UNIV,DEPT MOL BIOL,PROT RES GRP,CAMPUSVEJ 55 DK-5230 ODENSE M DENMARK ODENSE UNIV,DEPT MOL BIOL,PROT RES GRP DK-5230 ODENSE M DENMARK
Titolo Testata:
Journal of mass spectrometry.
fascicolo: 6, volume: 32, anno: 1997,
pagine: 593 - 601
SICI:
1076-5174(1997)32:6<593:MLDIMS>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
DESORPTION IONIZATION; 2,5-DIHYDROXYBENZOIC ACID; OPTIMIZATION; RESOLUTION;
Keywords:
MALDI-MS SAMPLE PREPARATION; MALDI-MS MATRICES; ON-TARGET SAMPLE PURIFICATION; ON-TARGET CHEMISTRY; MICROSCALE SAMPLE PURIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
20
Recensione:
Indirizzi per estratti:
Citazione:
M. Kussmann et al., "MATRIX-ASSISTED LASER DESORPTION IONIZATION MASS-SPECTROMETRY SAMPLE PREPARATION TECHNIQUES DESIGNED FOR VARIOUS PEPTIDE AND PROTEIN ANALYTES/", Journal of mass spectrometry., 32(6), 1997, pp. 593-601

Abstract

This study encompasses a collection of experiences with regard to numerous matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) sample preparation techniques in terms of their suitability for different peptide and protein analytes. Variants of both established and new sample preparation techniques for the MALDI-MS analysis of peptides and proteins are described. The importance of matrix selection, matrix and analyte concentration, pH adjustment, crystallization conditions and the use of additives is evaluated. The tolerance of the different sample preparations towards salts, buffers, synthetic polymers, detergents, denaturants and other contaminants, and also the influence of the preparation methods on undesired amino acid side-chain oxidation, are investigated. Moreover, the performance of on-target trypticdigestion and on-target disulfide reduction is shown and a microscalepurification procedure is described. According to this study, there is no universally applicable sample preparation for a broad variety of analytes. Rather, it is necessary to specifically adapt the sample preparation to the analyte properties. (C) 1997 by John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 03:14:03