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Titolo:
Actions of brain-derived neurotrophic factor on evoked and spontaneous EPSCs dissociate with maturation of neurones cultured from rat visual cortex
Autore:
Taniguchi, N; Takada, N; Kimura, F; Tsumoto, T;
Indirizzi:
Osaka Univ, Grad Sch Med D14, Biomed Res Ctr, Div Neurophysiol, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 physiol, Suita, Osaka 5650871, Japan Japan Sci & Technol Corp, CREST, Suita, Osaka 5650871, Japan Japan Sci & Technol Corp Suita Osaka Japan 5650871 , Osaka 5650871, Japan
Titolo Testata:
JOURNAL OF PHYSIOLOGY-LONDON
fascicolo: 3, volume: 527, anno: 2000,
pagine: 579 - 592
SICI:
0022-3751(20000915)527:3<579:AOBNFO>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
LONG-TERM POTENTIATION; TRUNCATED TRKB RECEPTORS; BDNF KNOCKOUT MICE; SYNAPTIC TRANSMISSION; HIPPOCAMPAL-NEURONS; SILENT SYNAPSES; CA1 REGION; PRESYNAPTIC ENHANCEMENT; MICROCULTURES; MODULATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Tsumoto, T Osaka Univ, Grad Sch Med D14, Biomed Res Ctr, Div Neurophysiol,2-2 Yamadaoka, Suita, Osaka 5650871, Japan Osaka Univ 2-2 Yamadaoka Suita Osaka Japan 5650871 50871, Japan
Citazione:
N. Taniguchi et al., "Actions of brain-derived neurotrophic factor on evoked and spontaneous EPSCs dissociate with maturation of neurones cultured from rat visual cortex", J PHYSL LON, 527(3), 2000, pp. 579-592

Abstract

1. To address the question of whether brain-derived neurotrophic factor (BDNF) directly enhances excitatory synaptic transmission, we recorded excitatory postsynaptic currents (EPSCs) from solitary neurones cultured on glialmicroislands for 7-38 days, and observed changes in EPSCs after the application of BDNF. In this preparation the possible action of BDNF on GABAergicinhibition was not involved, and evoked and spontaneous (miniature) EPSCs were derived from the same group of synapses (autapses).2. The application of BDNF at a concentration of 200 ng ml(-1) rapidly enhanced the frequency of miniature EPSCs (mEPSCs) in almost all the neurones tested. On the other hand, the amplitude of mEPSCs did not change at all, suggesting that the site of BDNF action is presynaptic.3. In contrast to the enhanced frequency of mEPSCs, evoked EPSCs were not potentiated in 61% of the cells tested. Most of these BDNF-insensitive EPSCs had a peak amplitude larger than 1 nA, while most of the other BDNF-sensitive EPSCs had a smaller amplitude. The former EPSCs had smaller coefficients of variation (CVs) of amplitude, while the latter had larger CVs, suggesting the possibility that the presynaptic release probability for the former groups of EPSCs might have beeen saturated so that the BDNF action was occluded.4. To test, this possibility we applied a low Ca2+ solution to 17 cells and reduced the amplitude of their evoked EPSCs to less than or near to 1 nA. It was found, however, that BDNF did not enhance these EPSCs. Rather, evoked EPSCs of almost all the cells cultured for less than 15 days were enhanced by BDNF, while those of most of the cells cultured for longer than 16 days were not enhanced.5. These results suggest that BDNF enhances transmitter release from presynaptic sites through its action on the release machinery, which can be differentiated into a BDNF-insensitive form for evoked release and a BDNF-sensitive form for spontaneous release with maturation of synapses.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 12:38:21