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Titolo:
Cholinergic neuronal modulation alters dopamine D-2 receptor availability in vivo by regulating receptor affinity induced by facilitated synaptic dopamine turnover: Positron emission tomography studies with microdialysis in the conscious monkey brain
Autore:
Tsukada, H; Harada, N; Nishiyama, S; Ohba, H; Kakiuchi, T;
Indirizzi:
Hamamatsu Photon KK, Cent Res Lab, Shizuoka 4348601, Japan Hamamatsu Photon KK Shizuoka Japan 4348601 Lab, Shizuoka 4348601, Japan
Titolo Testata:
JOURNAL OF NEUROSCIENCE
fascicolo: 18, volume: 20, anno: 2000,
pagine: 7067 - 7073
SICI:
0270-6474(20000915)20:18<7067:CNMADD>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
C-11 RACLOPRIDE BINDING; STRIATAL DOPAMINE; IN-VIVO; ENDOGENOUS DOPAMINE; L-TYROSINE; RAT-BRAIN; UNANESTHETIZED MONKEYS; INVIVO VOLTAMMETRY; N-METHYLSPIPERONE; CAUDATE-NUCLEUS;
Keywords:
L-[beta-C-11] DOPA; [C-11]raclopride; [beta-C-11]CFT; positron emission tomography; microdialysis; monkey brain;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Tsukada, H Hamamatsu Photon KK, Cent Res Lab, 5000 Hirakuchi, Shizuoka 4348601, Japan Hamamatsu Photon KK 5000 Hirakuchi Shizuoka Japan 4348601 apan
Citazione:
H. Tsukada et al., "Cholinergic neuronal modulation alters dopamine D-2 receptor availability in vivo by regulating receptor affinity induced by facilitated synaptic dopamine turnover: Positron emission tomography studies with microdialysis in the conscious monkey brain", J NEUROSC, 20(18), 2000, pp. 7067-7073

Abstract

To evaluate the cholinergic and dopaminergic neuronal interaction in the striatum, the effects of scopolamine, a muscarinic cholinergic antagonist, on the striatal dopaminergic system were evaluated multi-parametrically in the conscious monkey brain using high-resolution positron emission tomography in combination with microdialysis. L-3,4-Dihydroxyphenylalanine (L-[beta-C-11]DOPA) and 2 beta-carbomethoxy-3 beta-(4-fluorophenyl)tropane ([beta-C-11]CFT) were used to measure dopamine synthesis rate and dopamine transporter (DAT) availability, respectively. For assessment of dopamine D-2 receptor binding in vivo, [C-11]raclopride was applied because this labeled compound, which has relatively low affinity to dopamine D-2 receptors, was hypothesized to be sensitive to the striatal synaptic dopamine concentration. Systemic administration of scopolamine at doses of 10 and 100 mu g/kg dose-dependently increased both dopamine synthesis and DAT availability as measuredby L-[beta-C-11]DOPA and [beta-C-11]CFT, respectively. Scopolamine decreased the binding of [C-11]raclopride in a dose-dependent manner. Scopolamine induced no significant changes in dopamine concentration in the striatal extracellular fluid (ECF) as determined by microdialysis. However, scopolamine dose-dependently facilitated the striatal ECF dopamine induced by the DATinhibitor GBR12909 at a dose of 0.5 mg/kg. Scatchard plot analysis in vivoof [C-11]raclopride revealed that scopolamine reduced the apparent affinity of dopamine D-2 receptors. These results suggested that the inhibition ofmuscarinic cholinergic neuronal activity modulates dopamine turnover in the striatum by simultaneous enhancement of the dynamics of dopamine synthesis and DAT availability, resulting in no significant changes in apparent "static" ECF dopamine level but showing a decrease in [C-11]raclopride bindingin vivo attributable to the reduction of affinity of dopamine D-2 receptors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 18:39:14