Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Comparative localization of cathepsin B protein and activity in colorectalcancer
Autore:
Hazen, LGM; Bleeker, FE; Lauritzen, B; Bahns, S; Song, J; Jonker, A; Van Driel, BEM; Lyon, H; Hansen, U; Kohler, A; Van Noorden, CJF;
Indirizzi:
Univ Amsterdam, Acad Med Ctr, Dept Cell Biol & Histol, NL-1105 AZ Amsterdam, Netherlands Univ Amsterdam Amsterdam Netherlands NL-1105 AZ Z Amsterdam, Netherlands Alfred Wegener Inst, Dept Cell Biol & Pathol Biol Anstalt Helgoland, Hamburg, Germany Alfred Wegener Inst Hamburg Germany Anstalt Helgoland, Hamburg, Germany Univ Copenhagen, Kobenhavns Kommunes Hvidovre Hosp, Dept Pathol, Hvidovre,Denmark Univ Copenhagen Hvidovre Denmark re Hosp, Dept Pathol, Hvidovre,Denmark
Titolo Testata:
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
fascicolo: 10, volume: 48, anno: 2000,
pagine: 1421 - 1430
SICI:
0022-1554(200010)48:10<1421:CLOCBP>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-CELL-LINES; HUMAN LUNG; CYSTEINE PROTEINASES; PROGNOSTIC-SIGNIFICANCE; PLASMINOGEN-ACTIVATOR; MATRIX DEGRADATION; CYSTATIN-C; IN-VITRO; INVASION; CARCINOMA;
Keywords:
colorectal cancer; invasion; metastasis; cysteine proteinase; cathepsin B; immunohistochemistry; enzyme histochemistry; image analysis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
64
Recensione:
Indirizzi per estratti:
Indirizzo: Van Noorden, CJF Univ Amsterdam, Acad Med Ctr, Dept Cell Biol & Histol, Meibergdreef 15, NL-1105 AZ Amsterdam, Netherlands Univ Amsterdam Meibergdreef 15 Amsterdam Netherlands NL-1105 AZ
Citazione:
L.G.M. Hazen et al., "Comparative localization of cathepsin B protein and activity in colorectalcancer", J HIST CYTO, 48(10), 2000, pp. 1421-1430

Abstract

Cathepsin B is a lysosomal cysteine proteinase that may participate in cancer progression. We compared localization of its protein and activity during progression of human colorectal cancer. In adenomas and carcinomas, protein expression and, particularly, activity were elevated compared with thosein normal colorectal mucosa. In normal mucosa, cathepsin B protein expression was moderate in stroma and variable in epithelium, whereas activity wasmainly present in distinct areas of stroma directly underneath the surfaceof the colon and in epithelium at the surface of the colon. Stroma in adenomas and carcinomas contained moderate to high protein levels but little activity except for areas of angiogenesis, inflammation, and necrosis, in which activity was high. In adenomas and the majority of well-differentiated carcinomas and moderately differentiated carcinomas, cathepsin B protein andactivity were found in granular form in the epithelium, close to the basement membrane. Protein and activity levels were low and diffusely distributed in cancer cells in the remainder of the well-differentiated and moderately differentiated carcinomas and in all poorly differentiated carcinomas. Invasive fronts in most cancers contained moderate protein levels but high activity. We conclude that (a) activity localization is essential to understand the role of cathepsin B in cancels progression, and (b) cathepsin B activity in human colon is associated with invasion of cancer cells, endothelial cells, and inflammatory cells, and in cell death, both apoptotic and necrotic.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 19:27:03