Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Differential frequencies of p16(INK4a) promoter hypermethylation, p53 mutation, and K-ras mutation in exfoliative material mark the development of lung cancer in symptomatic chronic smokers
Autore:
Kersting, M; Friedl, C; Kraus, A; Behn, M; Pankow, W; Schuermann, M;
Indirizzi:
Univ Marburg, Zentrum Innere Med, Klinikum, Abt Hamatol Onkol Immunol, D-3550 Marburg, Germany Univ Marburg Marburg Germany D-3550 kol Immunol, D-3550 Marburg, Germany Krankenhaus Neukolln, Schwerpunkt Pneumonol Infektiol, Abt Innere Med 3, Berlin, Germany Krankenhaus Neukolln Berlin Germany , Abt Innere Med 3, Berlin, Germany
Titolo Testata:
JOURNAL OF CLINICAL ONCOLOGY
fascicolo: 18, volume: 18, anno: 2000,
pagine: 3221 - 3229
SICI:
0732-183X(20000915)18:18<3221:DFOPPH>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
BRONCHOALVEOLAR LAVAGE FLUID; ONCOGENE MUTATIONS; CLINICAL-SIGNIFICANCE; PROTEIN ACCUMULATION; GENE-MUTATIONS; EARLY EVENT; CELL-LINES; DIAGNOSIS; METHYLATION; DNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Schuermann, M Univ Marburg, Zentrum Innere Med, Baldingerstr, D-35033 Marburg, Germany Univ Marburg Baldingerstr Marburg Germany D-35033 , Germany
Citazione:
M. Kersting et al., "Differential frequencies of p16(INK4a) promoter hypermethylation, p53 mutation, and K-ras mutation in exfoliative material mark the development of lung cancer in symptomatic chronic smokers", J CL ONCOL, 18(18), 2000, pp. 3221-3229

Abstract

Purpose: The aim of this study was to investigate the frequency of three (epi)genetic alterations (p53 and K-ros mutations and p16(INK4a) promoter hypermethylation) in symptomatic chronic smokers compared with patients with lung cancer and to evaluate the use of exfoliative material for such analyses. Patients and Methods: Fifty-one patients with histologically confirmed lung cancer and 25 chronic smokers (> 20 pack-years) were investigated for mutations in the K-ras (codon 12) and p53 (codons 248, 249, and 273) genes andfor allelic hypermethylation of the p16(INK4a) gene. DNA was isolated fromsputum and bilateral bronchial lavage, and brushings were taken at bronchoscopy. Results: Forty-one genetic lesions were detected within exfoliative material from the group of 51 patients with lung cancer and 10 lesions in the chronic smoker group. K-ras mutations occurred exclusively in the lung cancer group, whereas p53 mutations and p16(INK4a) promoter hypermethylation were also found in chronic smokers. Three of eight chronic smokers who harbored an (epi)genetic alteration were subsequently diagnosed with lung cancer. Analysis of sputum yielded information equivalent to that of samples obtainedduring bronchoscopy. Conclusion: p16(INK4a) promoter hypermethylation and p53 mutations can occur in chronic smokers before any clinical evidence of neoplasia and may be indicative of an increased risk of developing lung cancer or of early disease. K-ras mutations occur exclusively in the presence of clinically detectable neoplastic transformation. Molecular analysis of sputum for such markers may provide an effective means of screening chronic smokers to enable earlier detection and therapeutic intervention of lung cancer. (C) 2000 by American Society of Clinical Oncology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 18:43:44